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. Changes in gene expression of B cell line Ramos RA-1 after infection with Francisella tularensis.
Matyášová, Simona ; Konečná, Klára (advisor) ; Kubelková, Klára (referee)
Francisella tularensis is a facultative intracellular bacterium causing disease tularemia of different species of mammals, including humans. It is able to infect phagocytotic as well as non-phagocytotic cells. We focused on the study of B cells, that role in the infection of F. tularensis have been, until recently, regarded as not significant. Over the last 10 years several studies showed important role of B cells in innate immunity to F.tularensis infection. The aim of this thesis was to idenify changes in the expression of genes that encode molecules of cytokines produced by B cells, after F. tularensis infection, due to stimulation with bacterial recombinant protein IglC and lipopolysaccharide. These changes were examinated on B cell line Ramos RA-1. Using reverse transcriptase polymerase chain reaction, we detected these cytokines: IFN-γ, IL-10 a TGF-β. Key words: Francisella tularensis, B cells, cytokines, reverse transcriptase polymerase chain reaction
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