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Histological and histochemical study of the male accessory glands mosquito Culex pipiens s.l. as that of model animal human prostate
 HODÍK, Jakub
ABSTRACT This thesis is a comparative histological study of accessory gonads (prostate) in model animal males. The animals compared included males of the mosquito species Culex pipiens s.l. as a representative of insects and males of the mouse as representatives of mammals. Furthermore, three staining methods applicable to histological examination of the prostate were compared. Histological and histochemical investigation of the mosquito was performed on its abdominal part or on its prepared gonads. The urinary bladder with the prostate was prepared from the male mouse. The tissue was fixed in Bouin{\crq}s fixative or in the Dubosque-Brasil modification. The fixed tissue was embedded in paraffin and sliced on a Leica rotary microtome. Two histological staining methods, viz. the Mallory method and the hematoxylin- eosin method, and one histochemical staining method, using paraldehyde-fuchsin light green orange G, were applied to the histological sections. Staining after Mallory appeared to suit best the comparative histological examinations. This technique provided the best picture of the tissues and cells in the two species studied. Hematoxylin-eosin staining, popular for mammal tissues, was not very suitable for insect tissues because the cells were not stained to a sufficient extent. As regards the staining of the male gonads with paraldehyde-fuchsin light green orange G, the result was non-representative for either species. The histological and histochemical comparative studies showed similar structures of the prostate and other organs of the male reproductive system. For insects to be usable as model animals, many investigations focussing particularly on immunohistochemistry and electron microscopy with immunolabelling or in situ hybridization will have to be performed.
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