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Enzyme tests with immobilized acetycholinesterase for the anti-Alzheimer's disease drugs characterization
Dulinová, Miriama ; Doležal, Martin (advisor) ; Zitko, Jan (referee)
Charles University, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical chemistry and drug control Consultant: prof. PharmDr. Martin Doležal, Ph.D. Supervisor specialist: pplk. prof. RNDr. Miroslav Pohanka, Ph.D., DSc. Student: Miriama Dulinová Title of diploma Thesis: Enzyme assays with bound cholinesterase to characterize drugs for Alzheimer's disease. Alzheimer's disease is a progressive and irreversible neurodegenerative brain disease, which is globally connected with the most common cause of dementia. The pathophysiology of the disease is not yet fully understood. Treatment of this disease is for the time being only symptomatic and is represented by inhibitors of the acetylcholinesterase. The acetylcholinesterase inhibitors are at present practically the only active substances that mitigate the effects of the Alzheimer's disease. In this thesis, we have investigated the ability to inhibit already known AChE inhibitors (rivastigmine, donepezil, galantamine, tacrine), that have been tested for their activity. We tested the effectiveness of the inhibitors with loose acetylcholinesterase (via the reference method) and subsequently with the immobilised acetylcholinesterase in clear gelatine, but also with added netted agents. (glutaraldehyd, EDC). For the purposes of our...
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