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SPE in steviol glycosides analysis
 Dalecká, Karolína ; Drastík, Martin (advisor) ; Kubíček, Vladimír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Karolína Dalecká Supervisor: Ing. Martin Drastík, Ph.D. Title: SPE in steviol glycosides analysis In the first part of this diploma thesis, the solid phase extraction method is described. The topics like the workflow, usability of sorbents and their formats and interactions with analytes are covered. The second part of this thesis is focused on utilization of solid phase extraction on samples containing steviol glycosides. An overview of solid phase sorbents their forms; solvents used for extraction and elution of steviol glycosides is provided in the discussion section at the very end of this work. Taking into account the fact that stevia has been used more and more on the European market in recent years, and is found in more food and other products, the frequency of determination of steviol glycosides and the use of solid phase extraction is on rise. The most commonly extracted samples include food products with steviol glycosides used as sweeteners or extracts of dried stevia leaves. The main sorbents used for solid phase extraction include silica gel with octadecyl and divinylbenzene-co-vinylpyrrolidine copolymer. This type of extraction is most often connected with high...
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