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Filter wheel for confocal microscope
Daňková, Barbora ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
The existing holographic Q-Phase microscope, located at the FME of Brno BUT, is planned to be expanded with a confocal fluorescence module based on digital micromirror device technology. This will be implemented instead of the existing epi-fluorescence module. For the existing solution of the module, it is necessary to design a fluorescent filter turret, which was missing in it. This work explains the basic techniques of confocal fluorescence microscopy and deals in more detail with multi-channel fluorescence confocal microscopy principles. An optical simulation of its illumination part is created to realise the exchanger's design. Thanks to it, the dimensional requirements for the optical components of the turret are determined. Commercially available solutions are also evaluated. A custom solution is proposed since their parameters do not meet the requirements. This includes the mechanical design based on several concepts as well as its robotization. The turret is designed for six fluorescent channels, and drawing documentation has been compiled. According to her, the turret was manufactured, assembled, and successfully tested in the last part of the work.
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Optimalization of Leishmania antigen formulations for the diagnosis of canine leishmaniasis.
Danková, Barbora ; Němečková, Ivana (advisor) ; Vokřál, Ivan (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Barbora Danková Supervisor of diploma thesis: RNDr. Ivana Němečková, Ph.D. Specialized Supervisor: Prof. Anabela Cordeiro da Silva Title of diploma thesis: Optimalization of Leishmania antigen formulations for the diagnosis of canine leishmaniasis Leishmaniasis is a vector-borne disease caused by a protozoan parasite of the genus Leishmania and is prevalent in 98 countries worldwide. Reliable and accurate tests are necessary for laboratory diagnosis of Leishmania infection because of the wide spectrum of clinical characteristics and symptoms and high rate of asymptomatic infections that may occure. Serological diagnosis of canine leishmaniasis (CanL), especially enzyme-linked immunosorbent assay (ELISA), proved to be usefull tool. Data obtained from ELISA assay for the detection of antibodies against selected recombinant A, B, C, D, E and F proteins were evaluated in infected canine sera from Portugal and Brazil. This work presents the best formulation of recombinant proteins for serological diagnosis. We found that antigen C showed high level of sensitivity and specificity in recognition of positive and asymptomatic sera infected with CanL. Key words: canine leishmaniasis,...
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Optimalization of Leishmania antigen formulations for the diagnosis of canine leishmaniasis.
Danková, Barbora ; Němečková, Ivana (advisor) ; Vokřál, Ivan (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Barbora Danková Supervisor of diploma thesis: RNDr. Ivana Němečková, Ph.D. Specialized Supervisor: Prof. Anabela Cordeiro da Silva Title of diploma thesis: Optimalization of Leishmania antigen formulations for the diagnosis of canine leishmaniasis Leishmaniasis is a vector-borne disease caused by a protozoan parasite of the genus Leishmania and is prevalent in 98 countries worldwide. Reliable and accurate tests are necessary for laboratory diagnosis of Leishmania infection because of the wide spectrum of clinical characteristics and symptoms and high rate of asymptomatic infections that may occure. Serological diagnosis of canine leishmaniasis (CanL), especially enzyme-linked immunosorbent assay (ELISA), proved to be usefull tool. Data obtained from ELISA assay for the detection of antibodies against selected recombinant A, B, C, D, E and F proteins were evaluated in infected canine sera from Portugal and Brazil. This work presents the best formulation of recombinant proteins for serological diagnosis. We found that antigen C showed high level of sensitivity and specificity in recognition of positive and asymptomatic sera infected with CanL. Key words: canine leishmaniasis,...
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Assortments of perennials used on the rural gardens in Pilsen region
Daňková, Barbora ; Matiska, Pavel (advisor) ; Ivana, Ivana (referee)
The thesis called Assortments of perennials used on rural gardens in the Pilsen region deals with using perennials in villages of Pilsen Region- Pilsen- North and Tachov with focusing
on rural gardens and partly on public green containing perennials in planting.
From the total number of 149 villages in observed regions about 103 villages were chosen and visited.
The thesis also focuses on characteristic of these plants. The rural gardens are analysed, too.
It deals with recommendation for using suitable perennials in given station and their next use in gardens and other places. The thesis also focuses on some parts of Pilsen Region, concretely on districts of Pilsen- North and Tachov. The observation of these districts took place from June to September in 2015. It describes these districts from the viewpoint
of climate, it means the average year temperature, rainfall and the altitude of both districts.
It concretely mentions chosen villages from the districts of North Pilsen and Tachov and defines villages in which was the green focused on timber species and grassy areas and villages where these areas were completed by floral decoration, especially by perennials.
It was found that in small villages were used 16, in middle villages 39 and in large villages
32 sorts of perennials. The largest number of sorts of perennials is used in middle villages (600-2000 inhabitants). In small villages (under the number of 600 inhabitants) perennials are planted mainly in the gardens of family houses and are missing in public places.
Among the most often planted perennials in the districts of North Pilsen and Tachov belong Aster dumosus, Bergenia crassifolia, Hemerocallis sp., Hosta sp., Iris sp., Phlox paniculata, Sedum spectabile, Sedum spurium, Yucca sp. and others.
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The Interaction between Psychological Stress and the Immune System
DAŇKOVÁ, Barbora
This work deals with the effect of undesirable stress on human organism and follows the responses of immunity system to long-term psychical burden. The goal is to provide a survey on this interaction and to alert to the burnout syndrome as manifestation of weakening of the organism at all its levels. The knowledge on the causes, symptoms and prevention of burnout syndrome is focused on the personality of the teacher whose profession is included among the most endangered ones. The results of the work confirmed the presence of the symptoms of burnout syndrome among teachers and alerted to the fact that stress is mostly present at the physical level or their perception. This work points out that the information on the manifestations of burnout syndrome is unquestionably included among the knowledge directed at efficient prevention.
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