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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Biologická charakteristika dendritických buněk
 Coufalová, Karmela
This thesis deals about characteristics of dendritic cells. Dendritic cells are antigen presenting cells that can stimulate naive T and B cells and they can regulate immune response of organism. They differ from other cells in that they can express MHC I and MHC II molecules and in that they do not have surface immunoglobulines or lymphocytic and monocytic characters. Dendritic cells are spread nearly through all tissues of the organism. There are two main pathways myeloid and lymphoid. Dendritic cells have two forms: immature (tolerogenic) and matture (immunoregulatory). Follicular dendritic cells are special case of dendritic cells. They are morphologically similar to dendritic cells, but they have not the same origin. Dendritic cells can represent vector for immunotherapy of allergic diseases, infections, autoimmune diseases, tumor immunotherapy and they can play role also in transplantology. Recently, research deals with antitumor vaccination.
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Izolace krevních monocytů skotu pro účely kultivace dendritických buněk
 Coufalová, Karmela
Monocytes are a population of mononuclear lekocytes. The aim of this thesis was to select a suitable methodology for the isolation of monocytes and their cultivation into dendritic cells. For the experiment, bovine blood was taken from vena jugularis externa. This blood was used to isolate monocytes based on the density gradient of OptiPrep solution and Histopaque solution. The results showed that the method of isolation based on the density gradient of the Histopaque solution and the magnetic separation appeared to be a more efficient method. The monocyte population was cultured for 72 hours. Cells were analyzed by light microscopy and CD14 positive cells appeared to be transformed into dendritic cells. The purity of these cell population was determined by flow cytometry. Dendritic cells have a wide range of utility mainly in immunotherapy of various diseases and also play an important role in cancer therapy.
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