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Senescence-associated secretory phenotype regulated by mTOR pathway in senescent cells
Maslyaeva, Sofya ; Ryšánek, David (advisor) ; Chrienová, Žofia (referee)
Senescence is a state of a cell characterized by permanent cell cycle arrest, growth of the cell, and other phenotypic changes, including proinflammatory secretome. Along with the beneficial aspects of senescence, it has an unfortunate outcome - it is a cause of age-related pathologies and tumor progression. Senecence-associated secretory phenotype (SASP) is responsible for that, as it alters the tissue microenvironment by the secretion of the inflammatory agents - chemokines, cytokines and other factors. Cellular senescence is linked to the activation of the mTOR (mechanistic target of rapamycin) nutrient- and mitogen-sensing pathway. Therefore, mTOR inhibition is a promising therapeutic strategy for the SASP suppression and of various types of cancer and age-related diseases. In this thesis I am going to summarize the current understanding of the mTOR's role in the mediation of SASP. Key words: Senescent cells, senescence-associated secretory phenotype, mTOR pathway, MTORC1
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Carbohydrate recognition of the novel colonization factor CS30 of enterotoxigenic E. coli
Chrienová, Žofia ; Kučerová, Marta (advisor) ; Miletín, Miroslav (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis University of Gothenburg Sahlgrenska Academy Institute of Biomedicine, Department of Medicinal Biochemistry and Cell Biology, Department of Microbiology and Immunology Student: Žofia Chrienová Supervisors: PharmDr. Marta Kučerová, Ph.D. Prof. Susann Teneberg, MD PhD Astrid von Mentzer, PhD Title of diploma thesis: Carbohydrate recognition of the novel colonization factor CS30 of enterotoxigenic E. coli Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in developing countries and travellers to these areas, as well as in many farm animals. The main ETEC virulence factors are two enterotoxins and outer membrane proteins called colonization factors that mediate the adherence of the bacteria to the host epithelial cell surface in the small intestine. Carbohydrate recognition of the recently identified colonization factor CS30 was characterised using binding studies with the mixture of glycosphingolipids isolated from the human and porcine intestine. The CS30 binding compound was identified as a mixture of sulfatides with different ceramide species by mass spectrometry and a binding assay with monoclonal antibodies directed towards SO3-3Galβ. Further testing...
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Carbohydrate recognition of the novel colonization factor CS30 of enterotoxigenic E. coli
Chrienová, Žofia ; Kučerová, Marta (advisor) ; Miletín, Miroslav (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis University of Gothenburg Sahlgrenska Academy Institute of Biomedicine, Department of Medicinal Biochemistry and Cell Biology, Department of Microbiology and Immunology Student: Žofia Chrienová Supervisors: PharmDr. Marta Kučerová, Ph.D. Prof. Susann Teneberg, MD PhD Astrid von Mentzer, PhD Title of diploma thesis: Carbohydrate recognition of the novel colonization factor CS30 of enterotoxigenic E. coli Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in developing countries and travellers to these areas, as well as in many farm animals. The main ETEC virulence factors are two enterotoxins and outer membrane proteins called colonization factors that mediate the adherence of the bacteria to the host epithelial cell surface in the small intestine. Carbohydrate recognition of the recently identified colonization factor CS30 was characterised using binding studies with the mixture of glycosphingolipids isolated from the human and porcine intestine. The CS30 binding compound was identified as a mixture of sulfatides with different ceramide species by mass spectrometry and a binding assay with monoclonal antibodies directed towards SO3-3Galβ. Further testing...
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