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Steric exclusion effects and biochemical activity of molecular motors
Brucknerová, Adéla ; Lánský, Zdeněk (advisor) ; Hendrych, Tomáš (referee)
Bachelor Thesis: Steric exclusion effects and biochemical activity of molecular motors Adéla Brucknerová, 2022 The cytoskeleton and its associated proteins, the so-called molecular motors, belong to the cellular apparatus of every eukaryotic cell. Molecular motors, including kinesin-1, have the ability to temporarily bind to the cytoskeleton and transport cargo vesicles (e.g. organelles) along it. During such movement, kinesin-1 molecules hydrolyse one ATP molecule for each step of their chemomechanical cycle. It turns out, that the chemomechanical cycle may be affected by the presence of inert macromolecules. The influence of these macromolecules on the chemomechanical cycle of kinesin-1 is, however, not entirely known. Using total internal reflection fluorescence microscopy, single molecules of kinesin-1 were tracked in vitro. Their movement along reconstituted microtubules was recorded and the average velocity was measured. The performed experiments were carried out with variety of concentrations of soluted macromolecules - polyethylene glycol. For experiments, polyethylene glycols of molecular weight 6 kg/mol, 1000 kg/mol and combination of both were used. From average velocities, biochemical activity of kinesin-1 molecules at various concentrations of polyethylene glycol was obtained. In...
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