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Humic substances as active or auxiliary pharmaceutical component
Blahuš, Petr ; Kalina, Michal (referee) ; Sedláček, Petr (advisor)
This thesis examines the effect of the potassium lignohumate on the transfer speeds of analgesic drugs through the synthetic Strat-M® membrane. In order to examine the transfer of these substances, vertical diffusion cells were used and the final samples were analysed using UV-VIS spectrometer and HLPC. The intention of this thesis was to optimise the process of preparing diffusion cells, to compare the transfer speeds of pure active substances and active substances with the potassium lignohumate, and to compare the permeability of Strat-M® membranes, by using pig skin membranes which were used in the previous study that this thesis continues from. The primary discovery was that the lignohumate slowed down the transfer of active substances through the membrane.
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Humic substances as modifiers of the transdermal absorption of active phasrmaceutical ingredients
Blahuš, Petr ; Klučáková, Martina (referee) ; Sedláček, Petr (advisor)
This thesis deals with the influence of humic substances on the transdermal penetration of the analgesic drug ibuprofen. Analgesics in the form of oral administration have major side effects. Thanks to topical preparations, side effects could be reduced and also bring a number of other benefits. The problem in the development of a transdermal form of the drug is the very effective barrier capacity of the human skin. Therefore, a lot of research is focused on the search for various accelerators that can improve its properties due to interaction with the drug. In the future, such substances could also include humic acids, which have a long tradition of use in a number of therapeutic approaches, and recently examples of their use in human medicine are increasingly appearing. The research was focused on the diffusive transport of sodium ibuprofen and its acidic counterpart. Franz diffusion cells were used to simulate the real environment, and skin isolated from pig ears was used as a membrane. Using UV-VIS spectrophotometry and HPLC, the samples were analyzed. Several values were calculated from the measured data, such as the effective diffusion coefficient, which were then compared and statistically checked. The result of this thesis is data that show the positive effect of humic substances on the transport of drugs through the skin membrane.
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Multilevel structural analysis of the attitudes toward physical education: Methodological considerations
Pecha, Ondřej ; Blahuš, Petr (advisor) ; Víšek, Jan Ámos (referee) ; Hagtvet, Knut (referee)
The present thesis introduces multilevel confirmatory factor analysis (MCFA) as an appropriate methodology to use when data are hierarchically structured and an identification of theoretical concepts of a given inventory is desired. The main purpose of this thesis is to introduce all possible methods and issues which are connected with attitude research. The MCFA methodology is demonstrated on the example of the attitudes toward physical education (ATPE). Data were collected from high school students (n = 1157) nested within PE classes (m = 87). A different factor structure of ATPE was determined at the student level (within-group) and at the PE class level (between-group). At the within-group level, Wear's four-factor theory of ATPE was confirmed. However, the existence of two wording factors (positive and negative) and the school PE-related factor was indicated. At the between-group level, the resulting three-factor model exhibited only a marginal fit. The structure of theoretical concepts and their indicators was indistinct, because the items were originally developed to measure ATPE at the within-group level. The analysis included four cases: (1) single-level continuous, (2) multilevel continuous, (3) single-level ordinal, and (4) multilevel ordinal. All four approaches were compared in terms...
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Humic substances as active or auxiliary pharmaceutical component
Blahuš, Petr ; Kalina, Michal (referee) ; Sedláček, Petr (advisor)
This thesis examines the effect of the potassium lignohumate on the transfer speeds of analgesic drugs through the synthetic Strat-M® membrane. In order to examine the transfer of these substances, vertical diffusion cells were used and the final samples were analysed using UV-VIS spectrometer and HLPC. The intention of this thesis was to optimise the process of preparing diffusion cells, to compare the transfer speeds of pure active substances and active substances with the potassium lignohumate, and to compare the permeability of Strat-M® membranes, by using pig skin membranes which were used in the previous study that this thesis continues from. The primary discovery was that the lignohumate slowed down the transfer of active substances through the membrane.
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|
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Multilevel structural analysis of the attitudes toward physical education: Methodological considerations
Pecha, Ondřej ; Blahuš, Petr (advisor) ; Víšek, Jan Ámos (referee) ; Hagtvet, Knut (referee)
The present thesis introduces multilevel confirmatory factor analysis (MCFA) as an appropriate methodology to use when data are hierarchically structured and an identification of theoretical concepts of a given inventory is desired. The main purpose of this thesis is to introduce all possible methods and issues which are connected with attitude research. The MCFA methodology is demonstrated on the example of the attitudes toward physical education (ATPE). Data were collected from high school students (n = 1157) nested within PE classes (m = 87). A different factor structure of ATPE was determined at the student level (within-group) and at the PE class level (between-group). At the within-group level, Wear's four-factor theory of ATPE was confirmed. However, the existence of two wording factors (positive and negative) and the school PE-related factor was indicated. At the between-group level, the resulting three-factor model exhibited only a marginal fit. The structure of theoretical concepts and their indicators was indistinct, because the items were originally developed to measure ATPE at the within-group level. The analysis included four cases: (1) single-level continuous, (2) multilevel continuous, (3) single-level ordinal, and (4) multilevel ordinal. All four approaches were compared in terms...
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