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Detection of functional disorders of platelets using the PFA device in comparison with conventional aggregometry
Klausová, Kateřina ; Bártů, Iva (advisor) ; Blažková, Hana (referee)
UNIVERZITA KARLOVA V PRAZE 2. lékařská fakulta Oddělení klinické hematologie ve FN Motol Kateřina Klausová Detection of functional disorders of platelets using the PFA device in comparison with conventional aggregometry Bachelor work Abstract The theme of this work is to compare two devices designed for measuring functional disorders of platelets - the PFA device and the agreggometer. The PFA device was introduced recently and measures closure time of capillary in a special cartridge. The measurement takes place in a whole blood sample. Aggregometer is used for stimulation of platelet aggregation by the inductors. The measurement takes place in platelet rich plasma. This work compares results of patients whose blood samples were measured on both devices. It figures out whether the results of the devices match and describes their advantages and disadvantages. The work leads to conclusion that is appropriate to use both devices for complementary results in the laboratory. Comparsion of patient samples demonstrates that patients with reduced percentage of aggregation have also prolonged capillary closure time.
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Comparing results of cell blood count and leukocyte differential count between different series of analyzers Sysmex
Opletalová, Monika ; Blažková, Hana (advisor) ; Bártů, Iva (referee)
In this bachelor thesis I focused on comparing results of cell blood count and leukocyte differential count between three types of Sysmex analyzers. XS - 1000 i, XT - 4000i and XE - 5000 are automatic analyzers, measuring on principles of flow cytometry and impedance methods using hydrodynamic focusation. These methods provide very accurate blood cell an alysis. The theoretical part of bachelor thesis is focused on description of hematopoesis, options for measuring cell blood counts and leukocyte differential counts and general description of measuring methods used by Sysmex analysers. In the experimental part I described the composition of mentioned analysers, their measuring channels and reagents which are used for peripheral blood cells analysis. Every sample from 50 patients was measured on each analyser and results were statistically compared by using ANOVA test. Graphs showing average values of each parameter for negative and positive patient ́s samples were made. The aim of this study was to summarize the main advantages and disadvantages of all three analyzers and determine whether differences in the results of samples between analyzers are statistically significant or not. Powered by TCPDF (www.tcpdf.org)
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Comparing results of cell blood count and leukocyte differential count between different series of analyzers Sysmex
Opletalová, Monika ; Blažková, Hana (advisor) ; Bártů, Iva (referee)
In this bachelor thesis I focused on comparing results of cell blood count and leukocyte differential count between three types of Sysmex analyzers. XS - 1000 i, XT - 4000i and XE - 5000 are automatic analyzers, measuring on principles of flow cytometry and impedance methods using hydrodynamic focusation. These methods provide very accurate blood cell an alysis. The theoretical part of bachelor thesis is focused on description of hematopoesis, options for measuring cell blood counts and leukocyte differential counts and general description of measuring methods used by Sysmex analysers. In the experimental part I described the composition of mentioned analysers, their measuring channels and reagents which are used for peripheral blood cells analysis. Every sample from 50 patients was measured on each analyser and results were statistically compared by using ANOVA test. Graphs showing average values of each parameter for negative and positive patient ́s samples were made. The aim of this study was to summarize the main advantages and disadvantages of all three analyzers and determine whether differences in the results of samples between analyzers are statistically significant or not. Powered by TCPDF (www.tcpdf.org)
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New antithrombotics and laboratory tests for monitoring their effect
Hadravová, Jiřina ; Bártů, Iva (advisor) ; Neupauerová, Jana (referee)
This bachelor work follows up the comparison of new antithrombotics with the existing commonly used drugs. The theoretical part describes the individual drugs, their characteristics, indications, advantages and disadvantages. The work presents a comparison of the need for routine laboratory monitoring of individual drugs. The practical part of this work describes methods for the determination of the existing and new drugs and compare them. The work contains the results of measurements of new antithrombotics by the individual methods of determination. I compared the measured results of the commonly used methods with methods designed directly for the determination of new antithrombotics and I evaluate which routine methods can be used to approximate assessment of new preparations. The samples from patients treated with anticoagulant therapy and healthy persons provided the Department of Clinical Hematology, University Hospital Motol in Prague. Powered by TCPDF (www.tcpdf.org)
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Detection of functional disorders of platelets using the PFA device in comparison with conventional aggregometry
Klausová, Kateřina ; Bártů, Iva (advisor) ; Blažková, Hana (referee)
UNIVERZITA KARLOVA V PRAZE 2. lékařská fakulta Oddělení klinické hematologie ve FN Motol Kateřina Klausová Detection of functional disorders of platelets using the PFA device in comparison with conventional aggregometry Bachelor work Abstract The theme of this work is to compare two devices designed for measuring functional disorders of platelets - the PFA device and the agreggometer. The PFA device was introduced recently and measures closure time of capillary in a special cartridge. The measurement takes place in a whole blood sample. Aggregometer is used for stimulation of platelet aggregation by the inductors. The measurement takes place in platelet rich plasma. This work compares results of patients whose blood samples were measured on both devices. It figures out whether the results of the devices match and describes their advantages and disadvantages. The work leads to conclusion that is appropriate to use both devices for complementary results in the laboratory. Comparsion of patient samples demonstrates that patients with reduced percentage of aggregation have also prolonged capillary closure time.
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A role of adipokinetic hormones in insect lipid metabolism
BÁRTŮ, Iva
This PhD. thesis summarizes the effect of adipokinetic hormones (AKHs) on a spectrum of mobilized lipids in model insect species the locust Locusta migratoria and the firebug Pyrrhocoris apterus. The results revealed that mobilization of diacylglycerols and fatty acids from the fat body into the haemolymph is not uniform and suggested there is partial specificity of individual AKHs. This could contribute to the answer of the question why some insect species have more than one AKH. The results of the thesis are supplemented by studies of AKH effect on lipid metabolism in stress conditions elicited by insecticide treatments. Co-application of AKH and insecticide enhanced the effect of insecticide
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Analysis of lipids mobilized by adipokinetic hormones in the firebug Pyrrhocoris apterur (Heteroptera: Pyrrhocoridae)
BÁRTŮ, Iva
An effect of two adipokinetic hormones (Pyrap-AKH and Peram-CAH-II) on distribution of diacylglycerol (DG) molecular species and their fatty acid (FA) constituents were investigated by liquid chromatography (HPLC) - electrospray ionization mass spectrometry (ESI-MS) in haemolymph of the firebug Pyrrhocoris apterus. The results show that DGs with characteristic FAs are preferentially mobilized from fat body (FB) by the action of both AKHs produced by P. apterus. Both the macropterous and brachypterous morphs show very close DG and FA profiles. A separate action of the Pyrap-AKH and the Peram-CAH-II, however, exerts distinct differences in FA distribution at the macropterous morph. It seems that partially C16 and mainly unsaturated C18 FAs play a dominant role in the AKH action with an exclusive position of linoleic acid (18:2) which represents 50 - 60% of the total DG mobilization. The metabolically active C16 and C18 FAs are preferentially absorbed from the linden seeds and accumulated in the FB.
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