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Metabolic pathways involved in the regulation of bioactive cytokinin levels in plants
Žižková, Eva
;
Motyka, Václav
(advisor) ;
Pernisová, Markéta
(referee) ;
Hoyerová, Klára
(referee)
SummarySummarySummarySummary Cytokinins (CKs) are important group of plant hormones involved in a wide range of physiological and developmental processes. Endogenous levels of CKs as well as proportions of individual CK forms and derivatives are not constant and differ among plant species. The amounts of biological active CK forms (free bases and ribosides) are regulated through tangled machinery of metabolic conversions including biosynthesis, conjugation and degradation pathways. The main object of this thesis was to characterize the metabolic pathways involved in the regulation of bioactive CK levels in plants especially via CK biosynthesis with aspect to the environmental stimuli and via N- glucosylation pathway. It was shown, that light signal is an important input for modulating some CK-related genes and CK levels in Arabidopsis plants. The complex diurnal expression profiles of CK-biosynthetic genes (AtIPT1 - AtIPT9) in Arabidopsis plants indicated a strong dependence of AtIPT1 and AtIPT5 on light/dark phase in leaf rossetes. In contrast, no diurnal oscillation of AtIPT transcript levels was recorded in roots. Although the content of endogenous CKs was not constant in plants and varied during a day, no statistically significant correlation between light/dark cycle and oscillation in CK levels was...
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Options of outdoor teaching environmental education in urban kindergarten Čéčova
ŽIŽKOVÁ, Eva
This thesis focuses on the environmental education of preschool children of ages 3 to 6 in outdoor places. The theoretical part describes the basic principles, contents and means used in environmental education in kindergartens as a cross-section theme of preschool educational programmes. It puts emphasis on outdoor forms of education which lead to practical introduction to the world around us and seek to awake the need to care for nature and the life in it, because these are essential parts of human environment. The chapter concerning the safety of environmental education is no less important. The practical part of this thesis focuses on preparation and description of particular options of outdoor environmental education in the busy parts of České Budějovice near MŠ Čéčova, and their application in accordance with the educational programme of this particular school.
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Total station Leica TC(R) 400 test.
ŽIŽKOVÁ, Eva
The objective of this thesis is to check the preciseness of the total station Leica TC(R) 407 ser. No.660021 by using the defined methods. The data collected through measurements and by the computation procedures according to the relevant standards and the proceedings described by the professional literature were compared with the data stated by the manufacturer. The accuracy of the telemeter and the goniometric part of the device was tested. The theoretical part of this thesis is focused on the basic measurement methods and distance determination, principle of the electronic direction measuring and the error theory. The practical part of the thesis is focused on checking the accuracy of the measured horizontal direction and the zenith angle, as well as checking the preciseness of the telemetric part of the device. As a result the standard deviations of the measured distances and directions have been determined.
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