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	Distribuce transpozonů na pohlavních chromozomech rostlin Šlapáková, Martina Transposable elements are mobile genetic units of DNA that comprise a major part of most plant and animal genome and play crucial roles in genome structure and evolution. Since transposable elements represent potentially harmful entities that can disrupt gene function via transposition, plants have developed epigenetic regulatory mechanisms based on small RNA molecules that mediate their transcriptional and post-transcriptional silencing. Although the basic features of this epigenetic regulation have been described, it is not clear yet in which manner the protective mechanism differ in the male and female germline pathways where transposon activity is the highest. Based on preliminary data obtained in the laboratory of the Department of Plant Developmental Genetics, a model describing preferential spread of some transposons in the male and other transposons in the female germline was created. The aim of the thesis is to confirm the model by fluorescence in situ hybridization (FISH) and quantitative signal analysis of most abundant LTR-retrotransposon families in dioecious Silene latifolia. The diploma thesis includes preparation of chromosome preparations, probe labelling, FISH, image analysis of acquired data and their statistical evaluation. Detailed record
	The significance of interaction and communication at dialyzed patients with application of using King´s nursing model ŠLAPÁKOVÁ, Martina Detailed record
	Význam glutathionu v hormonální regulaci odpovědi na stres u Arabidopsis thaliana Šlapáková, Martina Tripeptide glutathione plays a key role in combating stresses such as heavy metal or xenobi-otic exposure. Recent studies have shown that glutathione content is negatively modulated by cytokinin. Thus, glutathione may be intimately involved in enhanced stress tolerance displayed in cytokinin-deficient plants. To probe the physiological relevance of the crosstalk between cytokinin and glutathione, a response to a glutahione-depleting xenobiotic 1-chloro-2,4-dinitrobenzene (CDNB) was analysed in the model plant Arabidopsis thaliana. Here we show that CDNB causes decrease of cytokinin status. We found that transgenic plants with reduced endogenous cytokinin levels showed a CDNB-tolerant phenotype. Cy-tokinin depletion provoked the accumulation glutathione. Glutathione-deficiency abolishes CDNB tolerance of cytokinin-deficient plants supporting an essencial role of glutathione for CDNB sequestration. Our findings indicate that cytokinin is an important regulatory factor in plant adaptation to CDNB stress. Detailed record

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