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National Repository of Grey Literature

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Možnosti biotechnologií u konopí Šenkyřík, Josef Baltazar Theoretical part of the thesis Possibilities of Biotechnology for Cannabis deals with morphological, taxonomic and genetic characterization of Cannabis genus and presents an overview of biotechnological methods employed in scientific research of Cannabis. Aim of the experiment itself was to analyze genetic variability between particular genotypes of Cannabis. Six mixed Cannabis samples were analyzed using 8 microsatellite markers based on PCR. Twenty-three alleles with diameter of 2,88 per locus, whose size varied from 140 to 240 bp. The largest number of alleles was found in markers CAN0039 and CAN0093. Values of diversity index (DI), polymorphic information content (PIC) and probability index (PI) were calculated for each of the microsatellites and based on statistical evaluation, a dendrogram of similarity was determined. The results show applicability of microsatellite markers for analysis of genetic variability in Cannabis. Detailed record

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