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Transdermal in vitro permeation of methoxytacrine IV.
Režňáková, Eva ; Doležal, Pavel (advisor) ; Musilová, Marie (referee)
Rigorosal Thesis 2006, Eva Režnáková Abstract The theoretical section summarizes information about Alzheimer's disease and drugs used for therapy and possible ways the passage of drugs through the skin structure. In vitro permeation experiments using skin samples from excised from pig showed that the samples of 7-metoxytacrine dispersed in TRIS-buffer and propylene glycol yields average fluxes of substantially lower values than the permeation of 7-metoxytacrine from isopropyl myristate vehicle. It was also shown to affect the enhancing transkarbam 12 and both from hydrophilic and lipophilic of the environment. But the acceleration effect of pyrrolidone has not been proved. Azone and transkarbam 12 appears as promising permeation enhancers from isopropyl myristate donor vehicle. In the case of permeation from TRIS-buffer and propylene glycol mixture only transkarbam 12 appears to be suitable accelerant.
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Transdermal in vitro permeation of cyclosporine IV.
Krejčová, Hana ; Doležal, Pavel (advisor) ; Musilová, Marie (referee)
Rigorosal Thesis 2006, Hana Krejčová Abstract The theoretical section gives a brief overview of anatomy, physiology and function of the skin, transdermal formulations and options for increasing transdermal absorption of drugs. The main part of the chapter is focused on cyclosporine includes updated information published on knowledge of the human immune system, and immunosuppressants. Experimental work presents the results of in vitro permeation experiments on pig skin. Donor and acceptor phase was in most experiments consisting propylene glycol and Tris-buffer pH 7.1 at a 1:1 ratio. Only a few experiments have been used as the donor phase of 10% dispersion of CyA in isopropylmyristate. Permeant determination was performed by HPLC with UV detection at 220nm. As a donor samples were used in the supersaturated system without CyA Enhancer, then a 5% addition of DMI or arlasolve, pyrrolidone or transcutol, or 1% transkarbam 12. All enhancers in the used concentrations icreased a depot formation in the skin, transkarbam 12 in the lowest concentration. For transdermal in vitro permeation experiments CyA appears to be administered in more lipophilic donor phase. The ideal length of the permeation experiments, it appears time 15 hours.
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Transdermal electroporation of cyclosporine I.
Pavlík, Bohumil ; Doležal, Pavel (advisor) ; Musilová, Marie (referee)
Rigorosal Thesis 2006, Bohumil Pavlík Abstract Effective topical application of cyclosporine is considered to be therapeutically important in the treatment of psoriasis, however, it is complicated by barrier properties of cornified skin layers. Potentially promising approach to solving this problem is to use electroporation. Thesis provides a basic overview of the current state of knowledge in the field of transdermal electroporation. The experiment focuses on penetration testing of cyclosporine in the two pulse protocols. The difference between the application of two or ten rectangular pulses of 10ms duration at time 0 and 6 hours, at a distance of 30 seconds and the voltage pulse 450 is reflected only in the period up to 2 hours from the start of permeation, long-term effect was demonstrated. When verifying the importance of arrangement of electrodes there was no difference between the type of needle-and needle arrangement with a flat surface electrode observed.
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Transdermal in vitro permeation of terbinafine II.
Doubravová, Hana ; Doležal, Pavel (advisor) ; Musilová, Marie (referee)
Rigorosal Thesis 2006, Doubravová Hana ¨ ABSTRACT Thesis provides a detailed overview of the current perspective on the performance and state of stripping methods in the study of penetration of substances through the skin. Using the pre-certified protocol of stripping experiment were found that part of terbinafine penetrates through the epidermis to the dermis. For both single vehicle evaluated, the ispropylmyristate and isolane, this results are quantitatively comparable with those obtained for the preparation Lamisil cream. Arbitrarily chosen, the addition of 1 percent of transkarbam 12 in isopropylmyristate and/or isolane yielded in significant increase of amounts of terbinafine deposited in the dermis.
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Corrosion of biodegradable magnesium alloys in Hank's solutions
Faltejsek, Petr ; Fintová, Stanislava (referee) ; Doležal, Pavel (advisor)
The aim of the thesis was to design a methodology for in-situ evaluation of degradation of selected magnesium alloys using AFM in SBF solutions. Study of the degradation of magnesium alloys in a chemically different corrosive environments of simulated body fluid (SBF - Hank's solution). For the pilot study were used magnesium alloys AZ31 and AZ61, manufactured by advanced method of squeeze casting. Part of the thesis was to evaluate the influence of the chemical composition and structure on the degradation properties of these alloys.
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Preparation and properties of porous zinc material
Ryšťák, Jaroslav ; Fintová, Stanislava (referee) ; Doležal, Pavel (advisor)
Bachelor thesis is focused on preparation of bulk material prepared from zinc powder by cold pressing followed by sintering. Topic of the work includes characterization of prepared material depending on the choice of the compaction process conditions. Bulk material is characterized by physical-mechanical tests, structure and porosity. Thesis solving and focuses on study and control of processes during bulk material preparation and description of the processes from physically-chemical point of view of the structure creation and final material properties.
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Preparation and properties of bulk material from magnesium powder
Ruttkayová, Veronika ; Pacal, Bohumil (referee) ; Doležal, Pavel (advisor)
Diploma thesis focuses on preparation and characterization of bulk material prepared from pure magnesium powder. The topic includes all the parts of process of bulk material preparation, sintering at hot pressing, diffusion processes and structurally-mechanical properties with respect to final material porosity of the produced material. Thesis solving focuses on study and control of processes during bulk material preparation and description of the processes from physically-chemical point of view of the structure creation and final material properties.
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