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Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated
ŠTĚRBA, Ján
Glycosylation of four proteins from Borrelia burgdorferi s.s. was investigated ? flagellins FlaA, FlaB, and outer surface proteins OspA and OspB. Glycosylation of these four proteins was not proved by any of the used techniques. However, other glycan-staining positive proteins were present in the borrelia samples. These proteins were suggested to originate in the culture medium.
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History, analysis and list of manuscripts and old prints of the parish and dean library in Velké Meziříčí
MRŇA, Jaroslav
Parish libraries in the Czech and Moravian church province keep the indelible legacy of the lower clergy book culture. Despite their important mission these libraries still escape the interests of researches, ecclesiastical administrators and even the general public. Presented rigorous thesis Bibliotheca parochiae Magnomesericensi. History, analysis and list of manuscripts and old prints of the parish and dean library in Velké Meziříčí is therefore a contribution to this issue. The autor's goal was to arrange a part of the book collection and to present one of the forgotten durch libraries in Morava to the reader. The introduction presents shortly individual parts of the work with the criticism of used sources and literature, then four chapters succeed and the conclusion that evaluates the defined targets. The first chapter tells the historical story of the parish of Velké Meziříčí from the beginnings to the present days in the context of history of the same domain. The second chapter deals briefly with the history of church libraries in Morava focusing on the parish libraries. The history of book culture in the parish and history of the parish and dean library itself are the topics of the third chapter. In the last fourth chapter the list of manuscripts and old prints is analysed in terms of language, time, place, topic, edition and provenance. The final conclusion is followed by the list of used abbreviations, the list of used sources and literature and the supplements that include not only tables, graphs and photographs but also an extensive list of manuscripts and old prints with the commentary itself.
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Long-Term Cold Acclimation Extends Survival Time at 0°C and Modifies the Metabolomic Profiles of the Larvae of the Fruit Fly Drosophila melanogaster
KORBELOVÁ, Jaroslava
We assessed survival of larvae of the fruit fly, Drosophila melanogaster at 0°C after rearing them under three different acclimation regimes. A comparison of the larvae acclimated at constant 25°C with those acclimated at constant 15°C followed by constant 6°C for 2 d showed that long-term cold acclimation extended the lethal time for 50% of the population (Lt50) during exposure to constant 0°C as much as 630-fold (from 0.137 h to 86.658 h). Detailed metabolomic analyses showed that long-term cold acclimation modified the metabolomic profiles of the larvae. The most prominent responses were accumulations of proline (up to 17.7 mM) and trehalose (up to 36.5 mM). In addition, restructuring of the glycerophospholipid composition of biological membranes was observed.
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Poikilothermic traits in Mashona mole-rat (Fukomys darlingi). Reality or myth?
ZEMANOVÁ, Milada
The African mole-rats is a mammalian family well known for a variety of ecophysiological adaptations for strictly belowground life. The Mashona mole-rat (Fukomys darlingi) is supposed to have strong poikilothermic traits, because it is not able to maintain a stable body temperature at ambient temperatures below 20°C. In our study, we measured resting metabolic rate and body temperature of Mashona mole-rats across a gradient of ambient temperatures to test its poikilothermic traits. Tested mole-rats show typical mammalian pattern in resting metabolic rates. And their body temperature was stable at ambient temperatures from 10 to 25°C. We thus cannot confirm poikilothermic traits in this species.
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"Death, Death, you are always so carving of lives." Mortality and funeral ritual in Cheb in the second half of 19th century
KOLOUCHOVÁ, Jana
The presented thesis deals with the phenomenon of death in the second half of the 19th century using the area around the Cheb town as an example. The source base is made up by information from Christian registers of deaths, prescriptive regulations and tangible evidences. As a main methodological basis the Historical Demography was chosen, but the other approaches were used as a Historical Anthropology and Art History. The most important emphasis was oriented on getting results from statistical research. The index of death issues was researched from several circumstances. Not only the data on approximate life expectancy, infant mortality and seasonal movement was reached, but also the analysis of the causes of death was made with the respect of its German terms. The attention was oriented on infectious diseases, with which the society of the second half of 19th century was afflicted too. The question of spread of epidemics is given into the wider context in the subsequent chapter, which is devoted to influence to prevent the gradual lengthening of life expectancy. The attention was devoted to the analysis of preserved tombstones from the 2nd half of 19th century on the base of fieldwork. The artifacts were assessed from the point of view of artistic representation. The epitaphs were recorded as well for the identification of specific persons. The conclusion of this topic was the thought of vandalism of modern time and the importance of preservation of the testimonial of funeral culture as a proof of high art. The main aim of presented thesis is not only to find out what were the mortality situations of the monitored period, but also the changes of funeral rite influenced by emancipation of bourgeois society. The connection with early modern period tradition of colossal noble funeral ceremony was pointed out. The shift of attention from the deceased to the bereaved was substantiated on the analysis of the preserved tombstones from the second half of 19th century and from the parsing of the funeral announcements in the regional press. The complexity was amplified by the study of lives of the two generations of sculptors from Cheb, who influenced unchangeably the appearance of the local cemetery. Thanks to the language analysis of the term ?the Death? presented on the pages of Glossaries from that time and by the excursion into the folk?s habits connected with the end of life journey of rural population, the issues of Death and funeral ritual were studied from multiple perspectives. For obtaining the complete view, there was necessary to work not only with results of statistics, but also to consider the interdisciplinary approach.
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Transformation of the burgeois elites of town Slaný in the process of Counter-Reformation (1610-1635)
KADEŘÁBEK, Josef
The present thesis deals with the changes of municipal elite in the royal town of Slany in the first third of the 17th century. The research based on the analysis of urban books and church registers tries to show how the municipal elite formed in that period. It notices not only their own structures but also strategies that townsmen chose in an attempt to enter them. It also reflects the situation after 1620, when it attempts to show how the elite transformations occurred in the context of the ongoing Counter-Reformation. It takes into account the change of the legal status of the town and an extensive emigration of the late twenties. It asks whether there was a change of strategy in endeavours of common townsmen to join the municipal elite in Slaný at that time.
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