Original title: Alkaloidy kultivarů rodu Narcissus a jejich biologická aktivita I.
Translated title: Alkaloids of Narcissus species cultivars and their biological activity I.
Authors: Tkáčová, Beáta ; Hradiská Breiterová, Kateřina (advisor) ; Siatka, Tomáš (referee)
Document type: Rigorous theses
Year: 2024
Language: slo
Abstract: [eng] [cze]
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmaceutical Botany (16-16130) Author: Tkáčová Beáta Supervisor: PharmDr. Kateřina Breiterová, Ph.D. Title of thesis: Alkaloids of Narcissus species cultivars and their biological activity I. Key words: Narcissus, alkaloids, biological activity, Alzheimer's disease. The main aim of this diploma thesis was to obtain summary alkaloid extracts from bulbs of five species cultivars (Narcissus cv. Acropolis, Delta, White Marvel, Kedron, Scarlet Gem) and one variety (Narcissus albus var. plenus odoratus), labeled as AL-450, AL-457, AL-460, AL-463, AL-467 and AL-508, from which samples were subsequently prepared for GC-MS analysis and screening of biological activities (cholinesterase inhibitory activity and cytotoxicity). Totally, 18 alkaloids were identified by GC-MS analysis, comparing their mass spectra. Identified alkaloids include 3-O-demethylmaritidine, 11,12-dehydroanhydrolycorine, assoanine, dehydroassoanine, galanthamine, galanthine, haemanthamine, hippeastrine, incartine, lycoramine, lycorine, narwedine, norpluvine, pancracine, pluvine, pseudolycorine and tazettine. Samples of cultivars AL-457, AL-460, AL-463 and AL-508, showed relatively promising hAChE inhibitory activity in screening assay (c = 50 µg/ml),... Univerzita Karlova, Farmaceutická fakulta v Hradci Králové Ústav: Katedra farmaceutické botaniky (16-16130) Riešiteľ: Tkáčová Beáta Školitel: PharmDr. Kateřina Breiterová, Ph.D. Názov práce: Alkaloidy kultivárov rodu Narcissus a ich biologická aktivita I. Klúčové slová: Narcissus, alkaloidy, biologická aktivita, Alzheimerova choroba. Hlavným cieľom tejto diplomovej práce bolo získanie sumárnych alkaloidných extraktov z cibúľ piatich kultivárov (Narcissus cv. Acropolis, Delta, White Marvel, Kedron, Scarlet Gem) a jednej variety (Narcissus albus var. plenus odoratus), pod označením AL-450, AL-457, AL-460, AL-463, AL-467, AL-508, z ktorých boli následne pripravené vzorky pre GC-MS analýzu a screeningové stanovenie biologických aktivít (inhibícia cholínesteráz a cytotoxicita). Na základe GC-MS analýzy bolo identifikovaných celkovo 18 alkaloidov porovnávaním ich hmotnostných spektier. Medzi identifikované alkaloidy patria 3-O-demetylmaritidín, 11,12-dehydroanhydrolykorín, assoanín, dehydroassoanín, galantamín, galantín, hemantamín, hippeastrín, inkartín, lykoramín, lykorín, maritidín, narwedín, norpluvín, pankracín, pluvín, pseudolykorín a tazettín. Vzorky kultivárov AL-457, AL-460, AL-463 a AL-508 vykazovali pri screeningovom stanovení (c = 50 µg/ml) pomerne sľubnú inhibičnú aktivitu voči hAChE,...
Keywords: alkaloids; Alzheimer's disease; biological activity; Narcissus; Alzheimerova choroba; biologická aktivita; Narcissus. alkaloidy

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/211609

Permalink: http://www.nusl.cz/ntk/nusl-780977


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Rigorous theses