Original title: Izolace alkaloidů druhu Geissospermum vellosii Allemão a studium jejich biologické aktivity X.
Translated title: Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity X.
Authors: Jenis, Sebastián ; Šafratová, Marcela (advisor) ; Jenčo, Jaroslav (referee)
Document type: Rigorous theses
Year: 2026
Language: slo
Abstract: [eng] [cze]
Jenis, S: Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity X. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of pharmacognosy and pharmaceutical botany, Hradec Králové, 2025. Key words: Geissospermum vellosii, bark, alkaloidal extracts, isolation of alkaloids, GC/MS analysis, biological aktivity, enzyme activity. Geissospermum vellosii Alemão, an Amazonian representative of the Apocynaceae family, is known for its use in traditional medicine, particularly against malaria and other tropical diseases. The aim of this diploma thesis was the chemical analysis of fraction GV-10 obtained from column chromatography of the ether extract of the bark of this plant, with a focus on the identification of indole alkaloids. Thin-layer chromatography (TLC) was used for the preliminary screening of the presence of these compounds, which confirmed the presence of alkaloids. Using gas chromatography with mass spectrometry (GC-MS) and subsequently nuclear magnetic resonance (NMR), three major alkaloids were identified: norharman, normacusine B, and buchtienin, with the latter isolated compound representing a potentially novel molecule. Norharman had not previously been isolated from the genus Geissospermum, thus its... Jenis, S: Izolácia alkaloidov druhu Geissospermum vellosii Alemão a štúdium ich biologickej aktivity X. Diplomová práca, Univerzita Karlova, Farmaceutická fakulta v Hradci Králové, Katedra farmakognozie a farmaceutické botaniky, Hradec Králové, 2025. Kľúčové slová: Geissospermum vellosii, kôra, alkaloidové extrakty, izolácia alkaloidov, GC/MS analýza, biologická aktivita, enzýmová aktivita. Geissospermum vellosii Alemão, amazonský zástupca čeľade Apocynaceae, je známy svojím využitím v tradičnej medicíne, najmä proti malárii a ďalším tropickým ochoreniam. Cieľom tejto diplomovej práce bola chemická analýza frakcie GV-10 získanej zo stĺpcovej chromatografie éterového výtrepku z kôry tejto rastliny, so zameraním na identifikáciu indolových alkaloidov. Na predbežné sledovanie prítomnosti týchto zlúčenín bola použitá chromatografia na tenkej vrstve (TLC), ktorá potvrdila prítomnosť alkaloidov. Pomocou plynovej chromatografie spojenej s hmotnostnou spektrometriou (GC-MS) a následne pomocou nukleárnej magnetickej rezonancie (NMR) boli identifikované tri hlavné alkaloidy: norharman, normacusin B a buchtienin, pričom posledná izolovaná zlúčenina predstavuje potenciálne novú molekulu. Norharman nebol predtým izolovaný z rodu Geissospermum, jeho výskyt v tejto frakcii teda predstavuje nové zistenie....
Keywords: Geissospermum vellosii - bark - alkaloidal extracts - isolation of alkaloids - GC; MS analysis - biological aktivity - enzyme activity; Geissospermum vellosii - kůra - alkaloidní extrakty - izolace alkaloidů - GC; MS analýza - biologická aktivita - enzymová aktivita

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/211599

Permalink: http://www.nusl.cz/ntk/nusl-780960


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Academic theses (ETDs) > Rigorous theses