Role LRRK1 ve vývoji a funkci T-lymfocytů

Tomicová, Kateřina

LRRK1 is a multidomain kinase involved in intracellular trafficking and signaling, but its function in T cells is currently unknown. Previous data from our laboratory showed differential expression of Lrrk1 between early activated naive and memory CD8⁺ T cells, suggesting that LRRK1 may play a role in memory T-cell responses. The aim of this study was to determine whether LRRK1 contributes to T-cell homeostasis, T-cell development, and memory CD8⁺ T- cell responses. To address this question, we used several Lrrk1-deficient mouse models and combined steady-state phenotyping with adoptive transfer experiments and infection with Listeria monocytogenes expressing ovalbumin. We found that the absence of LRRK1 did not cause major changes in the composition of peripheral T-cell populations and did not substantially affect thymocyte development under steady-state conditions. We also examined a possible effect of LRRK1 deficiency on neutrophil homeostasis, but we did not confirm the increased neutrophil counts suggested by publicly available IMPC data. In addition, we investigated the role of LRRK1 in primary and secondary CD8⁺ T-cell responses. In both settings, LRRK1 deficiency did not affect donor-cell expansion, the distribution of memory subsets, or the expression of functional markers associated with...

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