Alkaloidy Croton linearis Jacq. (Euphorbiaceae) a jejich cytotoxická aktivita.

Talačová, Alexandra

1 ABSTRACT Talačová, A.: Croton linearis Jacq. (Euphorbiaceae) alkaloids and their cytotoxic activity. Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy and Pharmaceutical Botany, Hradec Králové 2026. Key words: Croton linearis, alkaloids, isolation, structural analysis, cytotoxicity This master's thesis was focused on the isolation of alkaloids from dichloromethane and chloroform-ethanol extracts obtained from the plant Croton linearis. Based on GC/LC-MS, NMR analysis, and optical rotation measurements, the isolated alkaloid was identified as (+)-glaucine. Despite repeated purification steps, it was not possible to isolate any other pure alkaloidal compounds from the chloroform-ethanol extract; only fractions weighing a few milligrams were obtained, which still contained a mixture of several substances. The use of the freely available CLC-Pred 2.0 server enabled the in silico prediction of the potential cytotoxic activity of glaucine against various cancer cell lines. Based on in vitro experimental approaches, it was demonstrated that (+)-glaucine does not exhibit significant cytotoxic activity against most of the tested cancer cell lines for the HeLa cell line, whereas the other tested lines did not show a relevant sensitive response. Compared to the reference...

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