Fenotypizace fibroblastů izolovaných z lidské hypertrofické a normální kožní jizvy

Futóová, Terézia

Pathological scarring, including hypertrophic scars (HTS), represents a severe fibroproliferative disorder driven by fibroblast dysregulation. Accurate in vitro modeling is essential for developing antifibrotic therapies, however, the stability of the scar phenotype during standard cultivation remains critically underexplored. The primary aim of this thesis was to characterize the phenotypic and functional heterogeneity of primary human dermal fibroblasts (HDFs) derived from HTS and normal scars (NS), and to critically evaluate the stability of their pathological imprint during standard 2D in vitro cultivation. Using a multi-parametric approach combining high-resolution flow cytometry and functional profiling, we demonstrated that HTS cultures are profoundly dominated by a reticular (CD90+) lineage, lacking the regenerative papillary subpopulation that remains present in normal scars. We identified a rapid, culture-induced phenotypic drift on rigid 2D substrates. The distinct pathological fingerprint (e.g., FAP expression, elevated collagen I production) was neutralized by passage 3. Furthermore, by statistically isolating the profound confounding effect of donor biological age using a multiple linear regression model (ANCOVA), our findings offer a critical perspective on current in vitro...

guest :: login Digital Repository
		Search		Submit		Help		About