Moderní přístupy k úpravě biologických vzorků před LC-MS analýzou

Reguli, Adam

Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Adam Reguli Supervisor: doc. PharmDr. Petra Štěrbová, Ph.D. Title: Advances in the sample preparation of biological matrices for LC-MS analysis Sample preparation is one of the most important steps in the LC-MS analysis of drugs in biological material. Conventional extraction techniques (protein precipitation, liquid-liquid extraction, solid-phase extraction) are traditionally used for this purpose; however, increasing emphasis is now placed on the use of more environmentally friendly microextraction methods. Another important trend is the miniaturization of sample collection, known as microsampling, which is used in both clinical practice and preclinical studies. This approach is less invasive, does not require trained personnel, and significantly reduces patient stress. In the case of dry microsamples, analyte stability is also improved, and sample transport is simplified. In preclinical studies, microsampling significantly reduces the number of animals needed for the study (especially small rodents such as mice), improves data quality, and is in line with the "3R" principle (refine, reduce, and replace). This dissertation thesis focuses on the development...

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