Original title: Dissecting the proton entry sites into mitochondrial ATP synthase of Trypanosoma brucei
Authors: SARAVANAN, Tivisha
Document type: Bachelor's theses
Year: 2025
Language: eng
Abstract: The project investigates the mechanistic advantage of the evolved front proton entry site blockage in mitochondrial ATP synthases of the flagellate protist Trypanosoma brucei. To study the effects of unblocking in both directions of ATP synthase activity, the subunit-aN of procyclic and bloodstream forms were modified using current gene editing tools. The stability of native mitochondrial protein complexes with respect to this genetic engineering was then assessed, highlighting the ways in which further experimentation with such essential genes can be enhanced.
Keywords: CRISPR/Cas9 double knockout; mitochondrial ATP synthase; proton entry sites; subunit-aN; Trypanosoma brucei
Citation: SARAVANAN, Tivisha. Dissecting the proton entry sites into mitochondrial ATP synthase of Trypanosoma brucei. České Budějovice, 2025. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/73593

Permalink: http://www.nusl.cz/ntk/nusl-695016


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses