Original title: Expression and purification of fibrinogen-related proteins from the soft tick Ornithodoros moubata
Authors: ACKERLAUER, Christina
Document type: Bachelor's theses
Year: 2024
Language: eng
Abstract: The significance of tick immune proteins is explored, specifically focusing on two fibrinogen-related proteins from the soft tick Ornithodoros moubata called Dorin M and OMFREP, which play a role in the tick's innate immune system. During this work, the genes of Dorin M and OMFREP were amplified through molecular cloning, before the proteins were produced in a bacterial expression system for the first time. After optimization of the expression procedure, the proteins were subsequently purified by Ni-NTA chromatography, and results were confirmed via western blotting.
Keywords: Dorin M; fibrinogen-related protein; OMFREP; Ornithodoros moubata; tick innate immunity
Citation: ACKERLAUER, Christina. Expression and purification of fibrinogen-related proteins from the soft tick Ornithodoros moubata. České Budějovice, 2024. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/59359

Permalink: http://www.nusl.cz/ntk/nusl-694355


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses