Úloha proteinů FcRL při oplození.

Šimková, Lucie

The interaction and fusion between sperm and oocyte membranes are facilitated by a diverse network of proteins found on the surface of both gametes. Numerous proteins responsible for gamete fusion have been identified, with key proteins present on both sperm and oocyte. Important fusion-related proteins include IZUMO1 on sperm membrane and JUNO on the oocyte membrane, called oolemma. These two proteins play crucial role in gamete recognition and binding. Additionally, members of tetraspanin family, are indirectly involved in membrane fusion and facilitate the process by organizing protein complexes and enhancing membrane interactions. Since JUNO is rapidly lost after the initial contact with IZUMO1, other proteins likely involved in the gamete fusion. The presence of Fc receptor-like 3 (FCRL3) on the human oolemma has been identified (Vondrakova et al., 2022) and further investigation is needed to understand its precise role and other possible integrations with other proteins, including the proteins of FCRL family. The aim of the thesis was to study the role of human Fc receptor-like 1 (FCRL1) in gamete binding and fusion through analysis of its possible interaction with FCRL3, JUNO and tetraspanins as well as IZUMO1 proteins. Transfected HEK293T cells were employed to express full-length proteins,...

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