Stanovení vybraných biomolekul v detritu používaném při odchovu kriticky ohrožené perlorodky říční

Kohút, Maximilián

In this bachelor's thesis, detritus from eight streams in the Aš and Šumava regions was studied, with samples collected three times during 2023. In bioassay, juvenile freshwater pearl mussels were fed with this detritus, and their growth rate was used as an indicator of detritus quality. To identify key parameters, the detritus was further characterized using several methods, some of which were carried out as part of this thesis. Detritus quality was evaluated based on amino acid content, alkaline phosphate activity and proteolytic activity. These parameters were compared with complementary methods and the growth rates of the mussels. Capillary electrophoresis allowed the quantification of 14 out of 20 proteinogenic amino acid. The total content of amino acids were only slightly lower across different sampling periods, while their relative proportions were highly consistent. These findings were compared with fluorimetric determination of primary amines, which revealed somewhat higher amounts of primary amines than amino acids. Zymography showed detritus samples from different sites exhibited no major differences in protease molecular profiles across sampling periods. However, overall proteolytic activity in the detritus did not correspond with the activity measured using fluorescently labelled...

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