Využití sub/superkritické fluidní chromatografie pro separaci syntetických kanabinoidů

Kapusta, Matúš

This bachelor thesis is focused on the evaluation of effects of various chromatographic conditions on retention and separation of eight synthetic cannabinoids in sub/supercritical fluid chromatography. The tested cannabinoids include UR-144, 5F-AKB48, 5C-AKB48, JWH-122, AM-694, AM-2201, MAM-2201 and 5F-MN-24. A partial objective of this thesis is to determine optimal conditions for separating these compounds using sub/supercritical fluid chromatography. The theoretical section outlines the principle of chromatographic separation. The following part provides more detailed overview of sub/supercritical fluid chromatography, including the instrumentation. Definition of synthetic cannabinoids, their classification, and effects on the human organism are also included. The experimental part is focused on evaluating the effects of varying chromatographic conditions on retention and separation. In this thesis polar Waters Torus™ DIOL column was used. The effects of the type and amount of the organic modifier in the mobile phase, as well as the separation temperature, on the retention and separation of the tested cannabinoids were evaluated. Additionally, the influence of basic additives, i.e. isopropylamine and diethylamine on chromatographic resolution, peak symmetry and on their retention was tested. The...

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