Original title: Hodnoceni endotelové dysfunkce v aortě u myšího modelu s predispozicí ke vzniku aterosklerózy.
Translated title: Evaluation of endothelial dysfunction in the aorta in a mouse model prone to atherosclerosis development.
Authors: Jevčák, Dávid ; Nachtigal, Petr (advisor) ; Tripská, Katarína (referee)
Document type: Master’s theses
Year: 2025
Language: slo
Abstract: [eng] [cze]
Dávid Jevčák Evaluation of endothelial dysfunction in aorta of mice model with atherosclerosis predisposition Diploma thesis Charles University, Faculty of Pharmacy in Hradec Králové Pharmacy Background: The aim of this diploma thesis was to investigate whether administration of a high-fat diet for 25 days in Leiden ApoE*3 mutation leads to a change in the expression of three significant markers of endothelial dysfunction, namely endoglin, ICAM-1 and VCAM-1, compared to a standard diet. Methods: Mice with the Leiden ApoE*3 mutation (n = 6, in each group) were used in the experiment: Leiden ApoE*3 chow mice, which were fed a standard diet for 25 days and Leiden ApoE*3 HFD mice, which were fed a high-fat diet for 25 days. Biochemical analysis was used to detect changes in cholesterol and triglyceride levels. Changes in the expression of endoglin, ICAM-1 and VCAM-1 were examined by Western blot analysis. Results: Surprisingly, administration of a high-fat diet for 25 days did not affect the expression of endoglin, ICAM-1 and VCAM-1 levels in Leiden ApoE*3 mice. Conclusion: No significant effect of high-fat diet in the Leiden ApoE*3 mutation on the altered expression of three significant markers of endothelial dysfunction, endoglin, ICAM-1 and VCAM-1, has been demonstrated, and further research is needed. Dávid Jevčák Hodnotenie endotelovej dysfunkcie v aorte u myšieho modelu s predispozíciou ku vzniku aterosklerózy Diplomová práca Univerzita Karlova, Farmaceutická fakulta v Hradci Králové Farmacie Cieľ práce: Cieľom diplomovej práce bolo zistiť či podávanie vysokotukovej diéty po dobu 25 dní u mutácie Leiden ApoE*3 vedie k zmene expresie troch významných markerov endotelovej dysfunkcie, a to endoglínu, ICAM-1 a VCAM-1, v porovnaní so štandardnou diétou. Metódy: V experimente boli použité myši s mutáciou Leiden ApoE*3 (n = 6, v každej skupine): Leiden ApoE*3 chow, ktorým bola podávaná po dobu 25 dní štandardná diéta a Leiden ApoE*3 HFD, ktorým bola po dobu 25 dní podávaná vysokotuková diéta. Na zistenie zmeny hladín cholesterolu a triacylglycerolov bola použitá biochemická analýza. Zmeny v expresii endoglínu, ICAM-1 a VCAM-1 boli skúmané pomocou Western blot analýzy. Výsledky: Podávanie vysokotukovej diéty po dobu 25 dní prekvapivo neovplyvnilo expresiu hladín endoglínu, ICAM-1 a VCAM-1 u myší s mutáciou Leiden ApoE*3. Záver: Nebol preukázaný signifikantný vplyv vysokotukovej diéty u mutácie Leiden ApoE*3 na zmenu expresie významných markerov endotelovej dysfunkcie endoglínu, ICAM-1 a VCAM-1 a je potrebný ďalší výskum.

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/198778

Permalink: http://www.nusl.cz/ntk/nusl-684781


The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses