Stabilita yohimbinu v historických léčivých přípravcích

Gubová, Hana

This bachelor thesis was focused on the determination of the stability of active ingredients and possible degradation products in historical medicinal preparations from the 20th century containing yohimbine and strychnine. These preparations were available in three different forms: injectable ampoules, dragees, and tablets. All of the preparations contained yohimbine, with the exception of the dragees, which were also contained strychnine. For this purpose, it was necessary to develop and optimize the RP-HPLC method with UV detection. The separation was performed on a XBridge BEH C18 column (150×3.0 mm; 2.5 m). Isocratic elution was used, where the aqueous component of two-component mobile phase was 0.1% aqueous formic acid, the organic component was methanol. Model preparation of possible degradation products of both yohimbine and strychnine by forced hydrolysis or oxidation was carried out. An HPLC-MS was used to analyze the products formed. Analysis of authentic Yohimbine Spofa ampoule samples showed degradation of yohimbine to yohimbic acid. The yohimbine content was found to be 52%, resp. 54%, of the declared amount. In the case of the solid forms of the historical medicinal preparations, the yohimbine content of Yohimbin AWD tablets was determined to be 86 %, while Yohimbin Heisler and...

guest :: login Digital Repository
		Search		Submit		Help		About