Original title:
CRISPR/Cas9 Genome editing in Pyrrhocoris apterus
Authors:
BERTOLUTTI, Maly Document type: Bachelor's theses
Year:
2019
Language:
eng Abstract:
The CRISPR/Cas9 technology is a cutting edge method to genetically modify specific targets in the genome, which allows to create mutants in non-model organisms. The aim of this project was to genetically modify the Pyrrhocoris apterus genomic sequence of the cryptochrome2 gene using the CRISPR/Cas9 technology. Specifically, this project aimed to create a mutant with removed or modified C-terminal part of the CRY2 protein, which should later allow for studying the role of this sequence in CRY2 function.
Keywords:
chryptochrome gene; ciradian clock; CRISPR technology; CRISPR/Cas9; CRY2; cryptochrome; entomology; genome editing; photoperiodic clock; Pyrrhocoris apterus Citation: BERTOLUTTI, Maly. CRISPR/Cas9 Genome editing in Pyrrhocoris apterus. České Budějovice, 2019. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice
(web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia. Original record: http://www.jcu.cz/vskp/53044