Original title:
Monitoring The Parameters Of Cells Migrating In Pseudo-3d Extracellular Matrix
Authors:
Zumberg, Inna Document type: Papers
Language:
cze Publisher:
Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií Abstract:
Cell migration plays an essential role in a number of biological processes, such as embryogenesis,the immune response, wound healing and inflammation. Most research regarding cellmigration is based on experiments using two-dimensional (2D) cell cultures, and the detailed molecularand biophysical mechanisms of these processes are already well known. However, muchless is known about cell behaviour in the three-dimensional (3D) environment of living tissues.Pseudo-three-dimensional (pseudo-3D) cell cultures bridge the gap between 2D and 3D geometriesand combine their advantages. This review presents the quantitative evaluation of human mesenchymalstem cells (hMSCs) migration parameters in pseudo-3D artificial extracellular matrix(ECM) of various compositions.
Keywords:
Collagen; Confocal microscopy; Extracellular matrix; hMSCs; Pseudo-3D Host item entry: Proceedings I of the 27st Conference STUDENT EEICT 2021: General papers, ISBN 978-80-214-5942-7
Institution: Brno University of Technology
(web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library. Original record: http://hdl.handle.net/11012/200769