Original title: Natural and Synthetic Components of Nucleic Acids as Reactive Sites for DNA Modification by Osmium Tetroxide Complexes
Authors: Havran, Luděk ; Špaček, Jan ; Rozkosna, Jana ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /35./, Jetřichovice (CZ), 20150518
Year: 2015
Language: eng
Abstract: Natural DNA electroactivity find wide use in electrochemical analysis of its interactions and damage. For some applications is useful applied redox active tag to improve specificity of the analysis. One from utilized labels in this field are complexes of osmium tetroxide with nitrogen ligands (Os, L), which produce with DNA electroactive covalent adducts. Prime reaction site for Os, L is C=C double bond in pyrimidine nucleobases. If is 2,2'-bipyridine used as ligand, reaction is specific for single strand DNA. In this contribution will be presented results of electrochemical analysis of Os, bipy adducts with different ODN containing chemically modified purine bases.
Keywords: dna; Electrochemical methods; Osmium tetroxide complexes
Project no.: GAP206/11/1638 (CEP), GA15-08434S (CEP)
Funding provider: GA ČR, GA ČR
Host item entry: XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference, ISBN 978-80-905221-3-8

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297901

Permalink: http://www.nusl.cz/ntk/nusl-396420


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