Original title: Electrochemical Analysis of Sybr Green I and its Interaction with DNA
Authors: Dudová, Zdenka ; Pivoňková, Hana ; Havran, Luděk ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /35./, Jetřichovice (CZ), 20150518
Year: 2015
Language: eng
Abstract: There are used many methods such real-time PCR and electrophoresis in molecular biology and biochemistry which are based on utilization of a fluorescent dye Sybr Green I (SG) for selective detection of double stranded (ds) DNA and its quantification. SG is a planar molecule (see Fig. 1) that intercalates into the DNA double helix and simultaneously can bind into minor groove of dsDNA. In our work we focused on electrochemical behavior of SG on a pyrolytic graphite electrode (PGE) using square-wave voltammetry (SWV). The voltammetric method was used in measurements of SG interactions with DNA at the PGE.
Keywords: gels; Pyrolytic graphite electrode; Square wave voltammetry; Sybr Green I
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference, ISBN 978-80-905221-3-8

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297896

Permalink: http://www.nusl.cz/ntk/nusl-396417


The record appears in these collections:
Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers