Original title: Role exocystu v obraně rostlin před patogenem
Translated title: Role of exocyst at plant pathogen defense
Authors: Sabol, Peter ; Kulich, Ivan (advisor) ; Šašek, Vladimír Matěj (referee)
Document type: Master’s theses
Year: 2012
Language: slo
Abstract: [eng] [cze]
Exocyst is a protein complex conserved in yeast, animals and plants. It mediates tethering of a secretory vesicle to the plasma membrane in the semifinal step of exocytosis. Several roles of exocyst in the processes of cell polarization in plant cells have been implied, including polarized growth of polen tubes and root hairs, cytokinesis, deposition of seed coat pectin and possibly autophagy. One of the most recent roles of exocyst includes also a response to bacterial and fungal pathogens. Exo70B2 and Exo70H1 subunits were shown to play prominent roles in this respect, with Exo70H1 being responsible for mediating defense against bacterial (Pseudomonas syringae) and Exo70B2 defense against both bacterial and fungal (Blumeria graminis) pathogens. Recently, new data appeared indicating the interaction between Exo70B1 and RIN4 and Exo70A1 and NOI6, respectively. RPM-1 interacting protein 4 (RIN4) is a well known negative regulator of both basal and effector-triggered resistance. This thesis shows interaction between NOI6 and several exocyst subunits, confirming previous data. I show here that exocyst subunints interact specifically with N terminus of NOI6 protein and that this interaction is lost in the shorter version of NOI6 mimicking AvrRpt2 cleavage. Since AvrRpt2 is an effector protein from... Exocyst je proteínový komplex konzervovaný v kvasinkách, živočíchoch a rastlinách. Sprostredkuje tethering (pútanie) sekretorického vezikulu k plazmatickej membráne v predposlednom kroku exocytózy. Boli naznačené viaceré úlohy exocystu v procesoch bunkovej polarizácie, vrátane polarizovaného rastu peľových vrecúšok a koreňových vláskov, cytokinézy, depozícii pektínu semenného obalu a pravdepodobne autofágie. Jedna z najnovších úloh exocystu zahrňuje tiež odpoveď na bakteriálne a hubové patogény. V tomto ohľade bola ukázaná hlavná úloha pre Exo70B2 a Exo70H1 podjednotky, pričom Exo70H1 je zodpovedný za sprostredkovanie obrany proti bakteriálnym (Pseudomonas syringae) a Exo70B2 za obranu proti bakteriálnym aj hubovým (Blumeria graminis) patogénom. Nedávno sa objavili nové dáta naznačujúce interakciu medzi Exo70B1 a RIN4 a resp. Exo70A1 a NOI6. RPM1 interagujúci proteín 4 (RIN4) je dobre známym negatívnym regulátorom bazálnej aj efektorom spustenej odolnosti. Táto práca ukazuje interakciu medzi NOI6 a viacerými podjednotkami exocystu, potvrdzujúc predchádzajúce dáta. Ukazujem tu, že podjednotky exocystu špecificky interagujú s N koncom NOI6 proteínu a že táto interakcia chýba v kratšej verzii NOI6 mimikujúcej AvrRpt2 štiepenie. Pretože AvrRpt2 je efektorový proteín z bakteriálneho patogéna Pseudomonas...
Keywords: arabidopsis; blumeria graminis; exocyst; polarized secretion; pseudomonas syringae; arabidopsis; exocyst; polarizovan8 sekrece; pseudomonas syringae;

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/41123

Permalink: http://www.nusl.cz/ntk/nusl-305425


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses