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Original title:
Vliv hluboké hypotermie na endokrinní funkci tukové tkáně
Translated title: The influence of deep hypothermia on the endocrine function of adipose tissue
Authors: Drápalová, Jana ; Haluzík, Martin (advisor) ; Flachs, Pavel (referee)
Document type: Master’s theses
Year: 2009
Language: cze
Abstract: Introduction and aims: Deep hypothermia is a medical method used in a variety of car- diological operations. Adipose tissue is one of the main endocrine organs and therefore its en- docrine function significantly influences the state of a patient. The aim of this study was to explore the influence of deep hypothermia on the expression of selected proteins in adipose tissue, which has yet to be described in scientific literature. Material and methods: Samples of subcutaneous and visceral fat were taken from 10 pa- tients, who underwent planned surgery for pulmonary hypertension. The samples were taken at the beginning and the end of hypothermia, and at the end of the operation, respctively. Real-time PCR method was used to determine relative gene expression of selected adipocy- tokines (BCL-2, GLUT1, GPX1, HIF1- , IL-6, IRS1, MCP1, MIF, MT3, TNF-) from total fat and isolated adipocytes. Results: Gene expression of GLUT1 (total subcutaneous fat), HIF1- (total subcutaneous and visceral fat) and IL-6 a MCP-1 (in all tissues) at the end of the operation was significantly higher compared to the gene expression at the beginning and end of hypothermia. Gene ex- pression of TNF- in total subcutaneous fat increased significantly at the end of the operation compared to the beginning of hypothermia. Gene...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/26115
Permalink: http://www.nusl.cz/ntk/nusl-281108
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
Translated title: The influence of deep hypothermia on the endocrine function of adipose tissue
Authors: Drápalová, Jana ; Haluzík, Martin (advisor) ; Flachs, Pavel (referee)
Document type: Master’s theses
Year: 2009
Language: cze
Abstract: Introduction and aims: Deep hypothermia is a medical method used in a variety of car- diological operations. Adipose tissue is one of the main endocrine organs and therefore its en- docrine function significantly influences the state of a patient. The aim of this study was to explore the influence of deep hypothermia on the expression of selected proteins in adipose tissue, which has yet to be described in scientific literature. Material and methods: Samples of subcutaneous and visceral fat were taken from 10 pa- tients, who underwent planned surgery for pulmonary hypertension. The samples were taken at the beginning and the end of hypothermia, and at the end of the operation, respctively. Real-time PCR method was used to determine relative gene expression of selected adipocy- tokines (BCL-2, GLUT1, GPX1, HIF1- , IL-6, IRS1, MCP1, MIF, MT3, TNF-) from total fat and isolated adipocytes. Results: Gene expression of GLUT1 (total subcutaneous fat), HIF1- (total subcutaneous and visceral fat) and IL-6 a MCP-1 (in all tissues) at the end of the operation was significantly higher compared to the gene expression at the beginning and end of hypothermia. Gene ex- pression of TNF- in total subcutaneous fat increased significantly at the end of the operation compared to the beginning of hypothermia. Gene...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/26115
Permalink: http://www.nusl.cz/ntk/nusl-281108
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
Record created 2017-04-25, last modified 2022-03-03