Název:
Direct coupling of supported liquid membrane extractions to capillary electrophoresis. Sensitivity enhancement in analyses of untreated complex samples
Autoři:
Pantůčková, Pavla ; Kubáň, Pavel ; Boček, Petr Typ dokumentu: Příspěvky z konference Konference/Akce: CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./, Brno (CZ), 2014-10-20 / 2014-10-22
Rok:
2014
Jazyk:
eng
Abstrakt: Extractions across supported liquid membrane (SLM) were in-line coupled to capillary electrophoresis (CE) commercial system for direct injection of basic drugs from raw complex samples. A micro-extraction unit was inserted into the CE system sampling device and ensured excellent sample clean-up and injections directly from the membrane surface. Transient isotachophoresis (t-ITP) combined with large electrokinetic injections of selectively extracted basic analytes increased the sensitivity of the analytical method up to 300-fold when compared with conventional hydrodynamic injections and capillary zone electrophoresis (CZE) of the basic analytes.
Klíčová slova:
capillary electrophoresis; supported liquid membrane; transient isotachophoresis Číslo projektu: GA13-05762S (CEP) Poskytovatel projektu: GA ČR Zdrojový dokument: CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-2-0