Original title:
Sample preparation for single cell analysis
Authors:
Adamová, Eva ; Basova, E. Y. ; Potáčová, Anna ; Foret, František ; Matalová, Eva ; Klepárník, Karel Document type: Papers Conference/Event: CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./, Brno (CZ), 2014-10-20 / 2014-10-22
Year:
2014
Language:
eng Abstract:
Development of sensitive, miniaturized and fast methods aims to reliable analyze even small amounts of samples. Micromanipulation systems such as laser capture microdissection enable to collect just single cells from cell suspensions or tissue samples. One option for further analysis is the bioluminescence reaction based on luciferin/luciferase conversion. This approach was used for detection of active caspases, caspase-3/7. Droplets based microfluidic systems provide benefits in biological, pharmaceutical and chemical research.
Keywords:
bioluminiscence; micromanipulation; single cell analysis Project no.: GA14-28254S (CEP), EE2.3.20.0182, GBP206/12/G014 (CEP) Funding provider: GA ČR, GA MŠk, GA ČR Host item entry: CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-2-0