Original title: Competitive incorporations of modified versus natural nucleotides by DNA polymerases
Authors: Kielkowski, Pavel ; Hocek, Michal
Document type: Papers
Conference/Event: Symposium on Chemistry of Nucleic Acid Components /16./, Český Krumlov (CZ), 2014-06-08 / 2014-06-13
Year: 2014
Language: eng
Abstract: A series of 7-substituted 7-deazaadenosine and 5-substituted cytosine triphosphates were tested in competitive incorporations with different DNA polymerases with their natural analogs and analyzed using restriction endonucleases and compared with steady state kinetics of the modified and natural dNTPs. Data sets correlation is up to 99 % and gave us to find modified dATPs with high incorporation ratios.
Keywords: DNA polymerases; dNTPs
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: Chemistry of Nucleic Acid Components. 16th Symposium, ISBN 978-80-86241-50-0

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0234288

Permalink: http://www.nusl.cz/ntk/nusl-174432


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers