Název:
Scavenger receptor - trypsinová peptidáza IrSRP-1 z klíštěte \kur{I. ricinus}
Překlad názvu:
Scavenger receptor - trypsine-like protease IrSRP1 from the tick \kur{Ixodes ricinus}
Autoři:
SINGEROVÁ, Barbora Typ dokumentu: Diplomové práce
Rok:
2013
Jazyk:
cze
Abstrakt: Scavenger receptors are a large family of structurally diverse molecules that have been implicated in a range of biological functions. In this work, a newly identified multi-domain scavenger receptor-serine protease IrSRP-1 from the tick Ixodes ricinus is characterized. IrSRP-1 is related to the serine protease Sp22D from the mosquito Anopheles gambiae. IrSRP-1 is expressed mainly in the tick gut but also in hemocytes, Malpighian tubules, tracheas and ovaries of fully fed females. This was confirmed with Western blots and immunohistological labeling with antibodies raised against recombinantly expressed IrSRP-1 trypsine-like domain. According to acquired qRT-PCR profiles relative expression of IrSRP-1 is strongly up-regulated during female feeding and remains unchanged in ticks experimentally injected with various microbes. Functional characterization by RNA interference revealed that lowering IrSRP1 expression leads to a higher mortality rate during tick female feeding.
Klíčová slova:
I. ricinus; scavenger receptor; serinová proteáza; I. ricinus; scavenger receptor; serine protease Citace: SINGEROVÁ, Barbora. Scavenger receptor - trypsinová peptidáza IrSRP-1 z klíštěte \kur{I. ricinus}. České Budějovice, 2013. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Instituce: Jihočeská univerzita v Českých Budějovicích
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Plný text je dostupný v digitálním repozitáři JČU. Původní záznam: http://www.jcu.cz/vskp/30786