Original title: Gene targeting in Silkworm (Bombyx mori) by Engineered Endonucleases
Translated title: Gene targeting in Silkworm (Bombyx mori) by Engineered Endonucleases
Authors: SAJWAN, Suresh Chandra Singh
Document type: Doctoral theses
Year: 2013
Language: cze
Abstract: This thesis describes the establishment of a precise gene targeting methodology in the silkworm Bombyx mori by technologies based on engineered endonucleases. Two classes of engineered endonucleases, ZFNs and full length TALENs were used for creating DSBs at specified sites in the colour marker genes (BmBlos2 and Bmwh3). Direct embryo microinjection of engineered nucleases mRNA were performed and let the nuclease proteins to disrupt the functions of these marker genes by creating DSBs and inducing error prone NHEJ mechanism. These experiments showed that both ZFNs and TALENs could be used for targeted gene disruption in silkworms.
Keywords: Bm1 element; BmBLOS2; Chimeric nucleases; Gene targeting; Lepidoptera; MMEJ; Nonhomologous end joining; ZFN; Bm1 element; BmBLOS2; Chimeric nucleases; Gene targeting; Lepidoptera; MMEJ; Nonhomologous end joining; ZFN
Citation: SAJWAN, Suresh Chandra Singh. Gene targeting in Silkworm (Bombyx mori) by Engineered Endonucleases. České Budějovice, 2013. disertační práce (Ph.D.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/13951

Permalink: http://www.nusl.cz/ntk/nusl-151839


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Doctoral theses