Original title: Příprava rekombinantních proteinů pro použití v tkáňových kulturách
Translated title: Preparation of racombinant proteins for use in tissue cultures
Authors: MIČULKOVÁ, Klára
Document type: Master’s theses
Year: 2012
Language: cze
Abstract: cDNA derived from exons 2 and 3 of the Ser2 (Sericin 2) gene of Bombyx mori and encoding 121 amino acids was expressed in Escherichia coli as a fusion protein with hexahistidine to allow purification by affinity chromatogramy on Ni-resine column. Several expression systems were used and the codon usage was optimized but the yield of recombinant protein remained low. Commercial sericin extract was therefore used in assay with human embryonic stem cells (hESC) ? the extract in some cases reduced defects (amplification of centrosomes) in hESC proliferating under in vitro conditions.
Citation: MIČULKOVÁ, Klára. Příprava rekombinantních proteinů pro použití v tkáňových kulturách. České Budějovice, 2012. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/30254

Permalink: http://www.nusl.cz/ntk/nusl-135818


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses