National Repository of Grey Literature 18 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
A simple method for detection of Czech bolting garlics (Allium sativum L.) using microsatellite analysis: methodology for practice
Ovesná, Jaroslava ; Mitrová, Katarína ; Kučera, Ladislav
The aim of the methodology was to verify and compile a set of microsatellite markers for the purpose of verification to be used for the undisputed identification of selected types of Czech bolting garlics. The object of submitted methodology is to simplify procedure that allow to determine Czech bolting garlic using microsatellite analysis (SSR- Single Sequence Repeats), which is applicable in testing laboratories. The described method allows to identify the variety and breeding material of bolting garlic of Czech origin using DNA markers (microsatellite alleles) that are present only for this group of varieties and breeding lines. By the amplification of the genome containing the microsatellite locus by polymerase chain reaction (PCR) using specific, fluorescently labeled primers, and subsequent analysis of the lengths of PCR products. Methodology newly brings a unique set of diagnostic SSR markers, describe the evaluation and interpretation of the results.
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Methodology for detecting the presence of cranberries and lingonberries in processed products using molecular SSR markers: methodology for practice
Kučera, Ladislav ; Ovesná, Jaroslava
The subject of the methodology is a procedure for diagnosing the presence of lingonberries (Vaccinium vitis-idaea L.) and cranberries (Vaccinum macrocarpon L.) in processed products such as tea or fruit ingredients of many food products (jams, jelly, purree, tea). The methodology exploit DNA sequences that are typical for each plant species. Thus, molecular DNA markers that characterize the plant species by SSRs (single sequence repeats) are used. The methodology describes the extraction of DNA from the assumed matrix, the use of selected SSR markers and the analytical data evaluation procedure. The method can be used in any solid equipped molecular-genetic laboratory. The method is applicable for the characterization of DNA isolated from any part of the plant, fruit, and derived food or food supplements. Method describes the procedure and necessary equipment to perform the analysis. Protocol recommends how to evaluate the results.
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Graffiti, street art and the specifics of the subculture
Popová, Sylva ; Grendelová, Erika (advisor) ; Chlubná, Jana (referee)
TITLE: Graffiti, Street art and specifics of subculture AUTOR: Sylva Popová DEPARTMENT: Department of Art Education SUPERVISOR: Mgr. Erika Grendelová ABSTRACT: The work is devoted to graffiti and its subculture in Prague. The work is focused on the old part of the town, where many monuments, buildings, walls, columns are marked by this art. The work presents graffiti in all its forms, both legal and illegal and tries to figure out where is the border between vandalism and art, then investigates the graffiti author's relationship to history, to other people's property, the attitudes of the population towards this subculture and the expression in spray. Keywords Subculture, authors, writer, graffiti, vandalism, art, sprays, markers, work.
Study of inflammation using epithelial cells
Majerová, Barbora ; Hodek, Petr (advisor) ; Koblihová, Jitka (referee)
The human respiratory system is in constant contact with heterogeneous agents from the environment. There must be effective mechanical lung barriers and sufficient immune protection due to continuous deposition of various substances in the respiratory system. The mutual balance between the mechanisms of natural and adaptive immunity of the lungs is essential for destruction of infectious agents without initiation of inflammatory response. Overreaction of the immune system of the lungs may lead to the production of various inflammatory mediators and cytokines such as interleukins IL-1, IL-6 and IL-18. When determining the immunogenicity of a substance, it has to be exposed to lung epithelial cells, and then the concentration of cytokines produced is measured. To determine the immunogenicity of mammalian immunoglobulin G and chicken immunoglobulin Y the subsequent twenty-four hour exposure to A549 lung cancer cell line was made. Concentration measurement of cytokines IL-1 and IL-6 was performed using Luminex method, which pointed out the immunogenicity of goat immunoglobulin G and certain chicken immunoglobulins Y.
Augmented User Interface
Vladík, Ondřej ; Beran, Vítězslav (referee) ; Najman, Pavel (advisor)
The purpose of this thesis is to design and implement an augmented user interface for widget management which uses a camera-projector system. Implement also includes a library of functions for semi-automatic calibration of this system. An interface to control the widgets using markers was designed. Various markers are arranged on the desktop (desk, wall or table) . The camera captures these markers in a camera image.The position of the markers in space is then determined from the camera image . Widgets are projected on the desktop next to the markers. User can freely move those markers and the widgets will follow the markers.
Augmented User Interface
Zahrádka, Jiří ; Beran, Vítězslav (referee) ; Španěl, Michal (advisor)
This thesis falls into a field of user interface design. It focuses on tangible user interfaces which utilize a camera and projector to augment physical objects with a digital information. It also includes description of calibration of those devices. The primary object of this thesis is the implementation of an augmented user interface for application windows management. The system consists of a stationary camera, overhead projector and movable tangible objects - boards. The boards are equipped with fiducial markers, in order to be tracked in a camera image. The projector displays the conventional desktop onto the table and the tangible objects. For example, application windows can be projected onto some boards, while the windows move and rotate simultaneously with the boards.
Eye Tracking During Interaction with a Screen
Pavelková, Alena ; Kajan, Rudolf (referee) ; Herout, Adam (advisor)
Tato práce popisuje systém pro sledování obrazovky počítače v reálném čase a určení pozice uživatelova pohledu na tuto obrazovku s využitím dat z eye-trackovacích brýlí a Uniform Marker Fields. Vytvořený systém lokalizuje obrazovku počítače ve snímcích z kamery umístěné na eye-trackeru snímající scénu před uživatelem. V této scéně je detekován marker zobrazený na monitoru počítače. Po jeho úspěšné detekci je marker skryt a ke sledování pozice obrazovky jsou dále využívány významné body detekované ve snímku z kamery ležící na obrazovce, případně z celého snímku. Pokud sledování významných bodů selže, marker je znovu zobrazen a systém je znovu inicializován pomocí detekovaného markeru. Aby byl marker pro uživatele co nejméně nápadný a obtěžující, jsou využity data z eye-trackeru k tomu, aby byl marker zobrazen co nejdále od oblasti uživatelova zájmu. Pro vyhodnocení výkonu a přesnosti vytvořeného systému a rušivosti markeru pro uživatele bylo provedeno několik experimentů a testování s uživateli.
Chosen problems of genetic diversity of dog
CHLUPATÁ, Lucie
The bachelor thesis is worked out in the form of the research on the theme "Selected questions from the genetic diversity of dogs". The aim of this work was processing of the current situation of scientific knowledge in the field of genetic diversity of dogs. Firstly, the origin is described in the thesis, domestication of dogs, an organisation dealing with dogs breeding and selected breeds of dogs. From the genetic site point of view, the basic terms in genetics are described genetic markers, DNA profile of dog and the methods of evaluation of genetic diversity. In the end of my bachelor thesis I outlined possible recommendations for dog breeders.
Genetic diversity of cattle in Czech Republic
ČERVINKA, Václav
The thesis deals with genetic diversity in the Czech Republic. Firstly domestication of livestock in general and then domestication of cattle is decribed. History of cattle in the Czech Republic, the importance of cattle breeding and last but not least the condition of cattle in the Czech Republic is also introduced. The thesis is also focused on biodiversity, threats to biodiversity and its conservation and the levels of diversity are also specified. Genetic markers,microsatellites, minisatellites, SNP, polymorphic structural genes and also mutations are described. The cattle breeds farmed in the Czech Republic are specified next. The National Programme, genetic resources and genetic resources in the Czech Republic are evaluated. Finally genetic resources of " česká červinka" and "český strakatý skot" are described.

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