National Repository of Grey Literature 81 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Nutriční a zdravotní aspekty konzumace tuků včetně cholesterolu
Kaňoková, Linda
In this bachelor thesis I mainly focused on the role of fats and oils along with cholesterol in human nutrition. At first I described nutrient composition of food, thus macronutrients and micronutrients and after describing those major food components I followed by describing lipids, where I predominantly focused on their most abundant part in food which consists of fats and oils. In this part I specified fatty acids and divided them according to the degree of their saturation. Next I described phospholipids and one of the accompanying substance of lipids, thus cholesterol, where I specified its natural occurence in food, metabolism, transport by lipoproteins and its importance in human body and nutrition. I also mentioned serious degenerative disease which can be caused by excess consumption of the wrong type of fatty acids. Last but not least I applied acquired knowledge to discuss the possibilities of influencing the level of total serum cholesterol and its HDL and LDL fraction by the type of consumed fatty acids and the amount of consumed cholesterol.
Vplyv prídavku vybraných olejov na zastúpenie mastných kyselín v živočíšnych tkanivách a homeostázu cholesterolu u modelového organizmu
Hnatová, Martina
The theoretical part of my thesis is based on the general classification and characterization of dietary lipids, especially fatty acids, cholesterol and lipoprotein particles. Also, their transport and absorption mechanisms are described, as well as medical complications that are closely linked to excessive lipid consumption. One part is used for the description of lipid extractions and determination of fatty acids. The experimental part is focused on the analysis of fatty acids in samples of liver, adipose and muscle tissues of laboratory rats after the addition of palm oil, safflower oil, fish oil and Schizochytrium microalgae oil into their diets. Lyophilized tissue samples were extracted and derivatized, followed by gas chromatography fatty acid determination. The results were then statistically evaluated.
Vztah SNP v genu pro leptin a parametrů vnitřního prostředí býků Českého strakatého skotu
Lysáková, Eva
There is a lot of factors which has impact on quality of bovine meat. The aim of this thesis was to examine correlations between chosen SNPs in leptin gene and specific hormones and metabolites, which have influence on the meat quality and also complete health condition of beef. There were 99 bulls of Czech Fleckvieh cattle used for this study. The examined parameters were insulin, glucose, cholesterol, triacylglycerol, non-esterified fatty acids and betahydroxybutiric acid. There was shown no statistically signifiant correlation between leptin gene polymorfisms and observed parameters. Only proved correlation was between leptin and cholesterol and glucose. There is lot of other factors involved in changes of amount of these substances though.
Study of interaction of negatively charged vesicular systems with polycations
Repová, Romana ; Klučáková, Martina (referee) ; Mravec, Filip (advisor)
This diploma thesis deals with the preparation and characterization of negatively charged catanionic vesicular systems and their combination with selected polycations. The catanionic vesicular system was prepared by mixing of two oppositely charged surfactants SDS and CTAB. The negative charge as well as the stability of the vesicular system was provided by the incorporation of phosphatidic acid. Polycations, DEAE and TMC, have been selected for use in a pharmaceutical applications. Characterization of the prepared systems was performed by measuring DLS and ELS. The results indicate that we were able to prepare stable negatively charged vesicles that were eligible to non-covalently interact with selected polycations.
Effect of weight reduction on blood lipids and some other risk factors of ischemic heart disease
Zdeňková Kroužková, Pavlína ; Zlatohlávek, Lukáš (advisor) ; Altschmiedová, Tereza (referee)
This bachelor thesis is focused on the effect of weight reduction on the level of total cholesterol, triglycerides, waist circumference and blood pressure. The effect of education, or changes in dietary and regime habits on weight, blood lipid levels (total cholesterol and triglycerides), waist circumference and blood pressure were monitored in a group of educated persons. The work is divided into two parts. The theoretical part contains information about obesity, ischemic heart disease, risk factors for these diseases, their prevention and therapy. The practical part of the bachelor's thesis is devoted to monitoring dedicated a group of patients who decided to lose weight by changing dietary and dietary habits. At baseline, patients were measured on by the Accutrend for total cholesterol, triglycerides, waist circumference, and blood pressure. The subjects were educated about the dietary regime and subsequently followed the recommended measures. Repeated measurements of all monitored indicators were performed after three months, when the effect of weight reduction on the monitored parameters was evaluated. After three months, all subjects demonstrated on average weight loss of 11.9 kg, BMI lowered by 3.94 kg / m2, and waist circumference parameters reduced on 8.9 cm. Systolic pressure decreased by...
Study of cholesterol depletion in human skin barrier
Audrlická, Pavla ; Vávrová, Kateřina (advisor) ; Nováková, Veronika (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Pavla Audrlická Supervisor: prof. PharmDr. Kateřina Vávrová, Ph.D. Title of diploma thesis: Study of cholesterol depletion in human skin barrier Barrier function of human skin barrier dwells in intercellular lipid membranes of the uppermost skin layer, the stratum corneum (SC), composed of equimolar mixture of ceramides (Cer), free fatty acids (FFA) and cholesterol (Chol).1 Chol is required for proper lipid organization of SC, however, it stays unclear, why is it present in an amount so high that it separates from other lipids.2 Experiments using synthetic model membranes with decreased Chol content suggested that molar ratio of Cer:FFA:Chol 1:1:0,4 is sufficient for lipid barrier formation and its complex functionality.3 The aim of this work was to manipulate Chol content directly in human SC and to study the effects of decreased Chol content on the SC permeability and microstructure. Ex vivo SC obtained from healthy donors was extracted by methyl-β-cyclodextrin (MCD) to reduce natural Chol content. The extracted SC did not show significant changes in Cer or FFA whilst the amount of Chol was lowered to 78 %. SC barrier properties were evaluated by measurements of transepidermal water loss...
Alternation of bile production due to iron depletion.
Šimková, Marie ; Prašnická, Alena (advisor) ; Hyršová, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Marie Šimková Supervisor: Mgr. Alena Prašnická Title of diploma thesis: Alternation of bile production due to iron depletion Introduction: Liver has an irreplaceable role in the production and secretion of bile. This body fluid serves as the main excretion way of some endogenous and exogenous substances. Another liver property is the ability to store substances essential for correct functions of the body, e.g. iron. It has been shown that iron could have an impact on the bile production and secretion. Aim: The aim of this diploma thesis was to discover an impact of iron depletion on the bile synthesis and metabolism, especially on bile acids, and the way it affects transporters expression. Methods: Male Wistar rats (n=6 in each group, 250 ± 20 g) were divided into two groups: control group (Chow diet) fed with standard diet and iron depletion group (ID), fed with iron depletion diet for 21 days. To investigate the changes in bile flow, the bile had been collected for 120 min during in vivo clearance study. The analysis of the changes in expression of bile transporters and enzymes responsible for de novo bile acid synthesis was performed at the mRNA (qRT-PCR) and protein (Western blot)...
Voltammetric behaviour of cholesterol on glassy carbon electrode
Bláhová, Eva ; Schwarzová, Karolina (advisor) ; Nesměrák, Karel (referee)
The aim of this Thesis is a study of voltammetric behaviour of cholesterol on glassy carbon electrode in the presence of acetonitrile and strong acid. The electrochemical oxidation occurs after dehydration of cholesterol induced by the acid. The study was performed using cyclic voltammetry, mostly in the presence of perchloric acid. Further, influence of other acids - sulphuric, nitric and phosphoric acid - was studied. Cholesterol provides in the presence of perchloric and sulphuric acid one anodic signal at the potential of +1400 mV vs. Ag/0,01 mol.l-1 AgNO3 in 1 mol.l-1 NaClO4 in acetonitrile. The influence of polarization rate, water content and concentration of perchloric acid in the supporting electrolyte on the oxidation signal of cholesterol was studied. Calibration dependence of cholesterol was measured using differential pulse voltammetry. The limit of detection was determined to be 4,83.10-6 mol.l-1 . Key words: Dehydration, glassy carbon electrode, cholesterol, oxidation, voltammetry
Alternation of bile production due to iron depletion.
Šimková, Marie ; Prašnická, Alena (advisor) ; Hyršová, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Marie Šimková Supervisor: Mgr. Alena Prašnická Title of diploma thesis: Alternation of bile production due to iron depletion Introduction: Liver has an irreplaceable role in the production and secretion of bile. This body fluid serves as the main excretion way of some endogenous and exogenous substances. Another liver property is the ability to store substances essential for correct functions of the body, e.g. iron. It has been shown that iron could have an impact on the bile production and secretion. Aim: The aim of this diploma thesis was to discover an impact of iron depletion on the bile synthesis and metabolism, especially on bile acids, and the way it affects transporters expression. Methods: Male Wistar rats (n=6 in each group, 250 ± 20 g) were divided into two groups: control group (Chow diet) fed with standard diet and iron depletion group (ID), fed with iron depletion diet for 21 days. To investigate the changes in bile flow, the bile had been collected for 120 min during in vivo clearance study. The analysis of the changes in expression of bile transporters and enzymes responsible for de novo bile acid synthesis was performed at the mRNA (qRT-PCR) and protein (Western blot)...
Endoglin and livers
Jozefčeková, Nikola ; Doleželová, Eva (advisor) ; Prašnická, Alena (referee)
Author: Nikola Jozefčeková Title: Endoglin and liver Form: Diploma Thesis University: Charles University, Faculty of Pharmacy in Hradec Králové Degree: Pharmacy This diploma thesis concludes the available information about endoglin, its isoforms in the liver and its impact on the liver during various pathological conditions. In the first part of the thesis describes morphology and physiology of the liver, its structure, histology and metabolic functions. Second part contains an information about endoglin, its isoforms and role in TGF-β signaling complex, expression of endoglin and regulation of the expression. In the third part are described liver diseases during which is expression of endoglin changed. This part deals with the significance of endoglin as a diagnostic and prognostic marker. Endoglin (CD105, TGF-β receptor III) is a homodimeric transmembrane glycoprotein and a membrane co-receptor TGF-β with high expression in endothelial cells. Endoglin plays an important role in the vascular development. Endoglin is involved in the processes of angiogenesis, vascular homeostasis and TGF-β signalization. It affects activity of TGF-βRII, ALK1 and ALK5 receptors. Due to the interactions with TGF-β complex, modulation of activity of ALK receptors and Smads, endoglin controls fibrotic and anti-...

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