|
|
Pallynological Analysis of Pollen Collected by Honey Bee (Apis mellifera) in the Prague-West District
Štrachová, Markéta ; Říhová, Dagmar (advisor) ; Hanel, Lubomír (referee)
Title of the Thesis The Palynological Analysis of the Pollen Collected by Honey Bee (Apis mellifera) in the Prague-West Area Abstract This diploma thesis underlines the importance and significance of bee pasture. The first part of the thesis contains the presentation of the honey bee (Apis mellifera). It also describes the structure of the pollen grain, pollen as such, and its use regarding bee nutrition, the factors determining bee harvest of pollen, the means of its collecting and the use of bee pollen and bee bread. Furthermore, there are characteristics of pollen pasture in the time of early spring, spring, summer, late summer and autumn. The second part is dedicated to the palynological analysis of the area of Prague-West, Úhonice. The diploma thesis contains the evaluation of the food offer for the bees from the aforementioned location, and the summary of pollen-yielding plants blooming between 1 April 2017 and 27 June 2017. The sampling of pollen balls, which had taken place within this time span, provided the initial information about the nutrition of bees as well as the data regarding the diversity of plant species in the chosen location. In total, the samples contained 98 kinds of plants, out of which 31 were dominantly present above the limit of 6 percent. The longest lasting source of pollen was...
|
|
|
The mechanisms of pollen incompatibility in the Brassicaceae family
Šesták, Petr ; Fíla, Jan (advisor) ; Vosolsobě, Stanislav (referee)
Sporophytic incompatibility (SI) represents one of the systems by which angiosperms prevent pollination by their own pollen or by the pollen from a genetically related plant. It is mostly studied in the Brassicaceae family, mainly due to its agricultural importance. Another reason is that the model plant Arabidopsis thaliana belongs to this family. In the last three decades, advances in molecular biological methods enabled the characterization of a large part of the signalling cascade that leads to the rejection of incompatible pollen. Then, the functions of various cellular components (e.g. cytoskeleton, exocyst or proteasome) involved in the incompatible response to pollination are studied mainly by live cell microscopy. Last but not least, the function of SI under various abiotic stresses was described to reveal their influence on SI mechanisms. The aim of this bachelor thesis is to summarize the new discoveries characterizing the molecular mechanisms of SI in the Brassicaceae family, to describe the processes leading to the germination of compatible pollen grain and to characterize the newly described proteins involved in cellular signalling leading to the rejection of incompatible pollen.
|
|
|
Pollens of Selected Alergenic Plants and their Didactic Use in Teaching Biology at High Schools
Pýchová, Adéla ; Skýbová, Jana (advisor) ; Říhová, Dagmar (referee)
In my thesis I deal with the pollen of allergenic plants, the origin and proces of allergy to plant pollen and the inclusion of these topics in the teaching of plant biology in high schools. In the theoretical part I focus on topic of pollen allergy. First, I generally describe allergy and its origin, causes of allergic diseases, environmental factors and the development of allergic disease. I also briefly address the naming and registration of allergens and I also mention the so-called cross-allergy. Great emphasis is placed on pollen allergy, which is devoted to a larger unit, divided into individual subchapters dealing with the physiological function of the nose, followed by an allergic reaction of type I, an allergic reaction that occurs on the nasal mucosa. Furthermore, subchapters mentioning the classification of allergic rhinitis, diagnosis and pharmacotherapy, including additions to the drug groups used in the treatment of pollen allergy. Another larger unit is devoted to the structure of the plant's blossom as a source of allergen, which includes the anatomy of the blossom parts and a description of the pollen grain. The anatomical description of the blossom is followed by a practical part, first created by a tabular overview with selected allergenic plants. Each plant causing the allergy...
|
|
|
Pollens of Selected Alergenic Plants and Their Didactic Use in Teaching Biology at High Schools
Pýchová, Adéla ; Skýbová, Jana (advisor) ; Říhová, Dagmar (referee)
7 Abstract The thesis is focused on the pollen of selected allergenic plants. Thesis is structured into two parts., pollination and fertilization , development of the pollen grain , its structure and chemical composition. The end of the theoretical part, dedicated to allergies, is divided into subsections dealing with First, teoretical part describes the flower and its morphology, discusses the pollen grain, stamen anatomical structure immunity and allergies, naming and registration of allergens and cross- pollen allergy . The practical part contains an analysis of curriculum for students of secondary schools. An integral part of the work is a survey of teachers in secondary schools, in which method of questioning was used. The aim of this questioning was to determine the presence of solved issue in terms of representation of pollen and pollen allergens in textbooks for secondary schools , as well as the inclusion of this issue in the official curriculum, under the National Programme for the Development of Education in the Czech Republic. Based on the results of a questionnaire survey of secondary school teachers, teaching materials have been developed in the form of two worksheets for teaching botany and guidance on laboratory exercises in plant biology.
|
|
|
Pallynological Analysis of Pollen Collected by Honey Bee (Apis mellifera) in the Prague-West District
Štrachová, Markéta ; Říhová, Dagmar (advisor) ; Hanel, Lubomír (referee)
Title of the Thesis The Palynological Analysis of the Pollen Collected by Honey Bee (Apis mellifera) in the Prague-West Area Abstract This diploma thesis underlines the importance and significance of bee pasture. The first part of the thesis contains the presentation of the honey bee (Apis mellifera). It also describes the structure of the pollen grain, pollen as such, and its use regarding bee nutrition, the factors determining bee harvest of pollen, the means of its collecting and the use of bee pollen and bee bread. Furthermore, there are characteristics of pollen pasture in the time of early spring, spring, summer, late summer and autumn. The second part is dedicated to the palynological analysis of the area of Prague-West, Úhonice. The diploma thesis contains the evaluation of the food offer for the bees from the aforementioned location, and the summary of pollen-yielding plants blooming between 1 April 2017 and 27 June 2017. The sampling of pollen balls, which had taken place within this time span, provided the initial information about the nutrition of bees as well as the data regarding the diversity of plant species in the chosen location. In total, the samples contained 98 kinds of plants, out of which 31 were dominantly present above the limit of 6 percent. The longest lasting source of pollen was...
|
|
|
Characterization of eukaryotic translation initiation factor 3 subunits (eIF3) in A. thaliana male gametophyte
Linhart, Filip ; Hafidh, Said (advisor) ; Retzer, Katarzyna (referee)
From RNA-to-protein, translation initiation and protein synthesis is mediated by trans-acting factors that recognize mRNA features common to almost all eukaryotes. Eukaryotic translation initiation factor 3 complex (eIF3) is a highly conserved protein complex that recognizes 5'-CAP elements of the mRNA to initiate translation. eIF3 consists of nine subunits, three of them having two isoforms: eIF3A, eIF2B1, eIF3B2, eIF3C1, eIF3C2, eIF3D, eIF3E, eIF3F, eIF3G1, eIF3G2, eIF3H and eIF3K. This work deals with functional characterization, expression and subcellular localization of eIF3B1, eIF3B2 and eIF3E in Arabidopsis thaliana male gametophyte and interaction of eIF3E with the Constitutive photomorphogenesis 9 (COP9) complex as a regulatory complex of eIF3E post-translational control. Here we show that depletion of eif3b1 or eif3b2 is not gametophytic lethal and that the two protein might function redundantly, whereas, knockout of eIF3E causes male gametophyte lethality. Interestingly, eif3b1 show post-fertilization defects during embryogenesis, suggesting that its redundancy with eIF3B2 is restricted to the gametophyte. Gene expression studies revealed high expression of eIF3 subunits in actively dividing zones of leaf primordia, root meristem and root elongation zones as well as in the vegetative...
|
|
|
Pollen charakterisation in size segregated aerosol
Kolpakova, Anna ; Hovorka, Jan (advisor) ; Zíková, Naděžda (referee)
This bachelor thesis, in literature review, deals with bioaerosol issue, describes meaning of pollen, ways of pollen spreading and its influence on human health, summarize methods of pollen sampling from the air and common ways of pollen grains identification. In the experimental part, the use of the first stage of a High Volumetric Cascade Impactor (Hi-Vol) BGI-900 for bioaerosol sampling and subsequent SEM analysis was proved. Method of pollen separation from the impaction substrate, polyurethane foam, into homogeneous deposit on Nylon filter was optimized. Representative portion of the deposit was analyzed by SEM. There were taken 485 SEM pictures from 12 samples in 3 localities in the ČR. Pollen grains were identified in 295 SEM and determined into 9 genus and 4 families and grain deformities were quantified to be 24, 18, 50% for Prague, Brezno and Láz localities. Number of pollen increased with total aerosol mass in Prague locality only. Thre aleo identified insect secrction products borochomomes in the samples. Key words: pollen, pollen grain, bioaerosol, cascade impactor, SEM.
|
guest ::
