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Využití přípravků umožňujících kontrolované uvolňování hormonální látky v umělé reprodukci ryb
PECHA, Oldřich
This bachelor thesis was focused on identification of species-optimized preparations based on PLGA microparticles in terms of length and intensity of release and composition of active substance (GnRHa, metoclopramide), which would be used in artificial reproduction of selected fish species - pike (Esox lucius). Under the conditions of the Experimental Fish Farm, FROV JČU in Vodňany and the Tábor Štičí líheň ESOX s.r.o. the first two-week experiment was conducted to achieve the desired results, which were achieved with males of pike. A year later, the experiment took place again in Tabor and newly in the hatchery in Mydlovary. This time we focused on females of pike. During the first research, we have achieved satisfactory results and confirmed that we are going in the right direction. Combination of GnRH with dopamine antagonist Metoclopramide has been shown to be most effective. The best results were achieved by application of 20?g GnRHa.kg-1 + 20mg MET.kg-1, where we achieved positive results in all parameters - increased milt production volume, increased sperm motility and speed, decreased 11-ketotestosterone concentration. During the second experiment with female, we showed that the combination of GnRH + MET at a concentration of 20?g GnRHa.kg-1 + 20mg MET.kg-1 has a positive effect on ovulation achievement, latency time reduction and higher concentration of 40?g GnRHa.kg-1 + 20 mg MET.kg-1 also for fertilization and hatching of eggs, but at the cost of lower fecundity. The results of the experiments show that by using this combination and the correct concentration of GnRH with the dopamine antagonist it is possible to induce maturation of the gametes of male as well as female of pike fish. However, there is still room for improvement of female.
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