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The effect of Bisphenol S on porcine oocyte meiotic maturation
Kosařová, Zuzana ; Petr, Jaroslav (advisor) ; Kaňka, Jiří (referee)
Meiotické zrání je klíčovou fází vývoje samičích gamet. Do regulace procesu zrání mohou negativně zasahovat látky, které vstupují do organismu z vnějšího prostředí a následně narušují homeostázu na úrovni hormonálního řízení. Tyto látky se označují jako endokrinní disruptory (ED). Mezi nejrozšířenější ED patří bisfenoly. Po prokázání ED aktivity a právních regulacích využití bisfenolu A značně stoupla světová produkce bisfenolu S (BPS). Recentní studie potvrzují negativní vliv BPS přítomného v organismu na reprodukci lidí a zvířat. Přesto zůstává řada dopadů BPS na oogenezi stále nejasná. Tato práce je zaměřena na hodnocení vlivu BPS na vybrané markery rozpadu jaderné membrány v průběhu rozpadu zárodečného váčku (lamin A/C, lamin B1 a LEMD3) a na přeskupování chromatinu po výstupu prasečích oocytů z I. meiotického bloku. Výsledky prokazují negativní vliv BPS na průběh meiotického zrání zrychlením či zpomalením dějů, které souvisejí s rozpadem jaderné membrány a na nutnost se tímto tématem dále zabývat. Klíčová slova endokrinní disruptor, bisfenol S, meiotické zrání, rozpad jaderné membrány, oocyt
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Dopad alimentární expozice bisfenolu S na vybrané orgány laboratorních potkanů
ROČEŇOVÁ, Adéla
The present thesis investigated the impact of a ten-week oral exposure to BPS (50 ?g/kg live weight/day) in female outbred Wistar Han rats. The effect of BPS exposure on live weight, absolute and relative kidney and liver weights, morphometric parameters of kidney and renal corpuscles, histopathological changes of kidney and liver were evaluated. In addition, the concentration of total protein, triacylglycerols, cholesterol, urea and alanine aminotransferase activity were determined in blood serum.
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Dopad různé alimentární expozice bisfenolu na játra potkanů
LABUDOVÁ, Kateřina
Bisphenol S (BPS) is an organic compound widely used in industrial production as a substitute for bisphenol A (BPA) for which negative impacts on the human body have been demonstrated and have therefore been significantly regulated. However, as time progresses, the negative properties of BPS are also shown, especially for the endocrine and reproductive systems. The liver is an organ in which xenobiotics are detoxified and thus BPS and BPA. The aim of the bachelor thesis was to verify whether 10-week oral exposure to BPS (4 g, 50 g, 100 mg/kg bw/day) and BPA (100 mg/kg bw/day) will lead to a negative impact on body weight, weight gain, weight and morphological liver structure and on the concentration of triglycerides, cholesterol, total white-vinas, urea and alkaline phosphatase activity in blood serum. The experiment involved 24 rats. BPA and BPS were administered daily by gastric tube in sunflower oil. The results of our research clearly did not show a negative effect of BPS and BPA in rats on body weight, absolute gain, absolute and relative liver weight in rats. There was also no evidence of histopathological changes in rat liver. In contrast, bio-chemical parameters were altered compared to the control group. Decreases in trigly-cerides, total protein content, urea, alkaline phosphatase activity and increases in serum cholesterol were noted. In the 50 g/kg body weight/day group of rats, the highest and lowest decreases in biochemical parameters were observed, respectively. The exposure dose of 100 mg BPS and BPA in our experiment did not cause liver histopathological changes or changes in biochemical parameters according to the hy-pothesis. A possible explanation could be given by the carrier for which sunflower oil with natural antioxidant content was used.
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Účinek bisfenolu S na vybrané krevní parametry potkanů
HLATKÁ, Tereza
Bisphenol S (BPS) is an organic substance which has replaced significantly industrially used bisfenol A (BPA). Eventhough BPS shows higher tolerance against physical influence, there have been registered leaks from the final products and there is a posibility of wather, food and environtment contamination. Until now the BPS has been, just like BPA, mostly connected with disruption of the reproductive system of human and animals. The aim of the bachelor thesis was to determinate whether a ten weeks oral exposure to BPS (50 ?g, 100 mg/kg live weight/day) leads to disturbance in selected haematological parameters (erythrocyte count (RBC), haemoglobin concentration (HGB), haematocrit value (HCT), mean corpuscular volume (MCV), erythrocyte haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH), total leucocytes, percentage of granulocytes, lymphocytes and monocytes). Eighteen Wistar Han rats were enrolled in the experiment and they were divided into three groups (control group, 50 ?g BPS, 100 mg BPS). Dissolved BPS in sunflower oil was given to the experimental groupts by a gavage. Blood was collected through cardiac puncture and analyzed using an automated hematology analyser. In the particular study, we found out a nonsignificant decrease of HGB and nonsignificant increase of MCV and MCH in experimental animals. BPS also led to a nonsignificant changes in the number of leukocytes (increase in the total number of leukocytes) and it led to a changes in the number of individual types of the white blood cells representation (increase in the percentage of granulocytes, decrease in lymphocytes). More significant effect was observed in 50 ?g BPS group. Our results did not conclusively proved the negative effect of BPS on the selected blood parameters of rats.
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Bisphenol S influence on selected markers of meiotic maturation of porcine oocytes
Černíková, Terezie ; Petr, Jaroslav (advisor) ; Krylov, Vladimír (referee)
Bisphenol A is a widely used chemical in the manufacture of plastics. The presence of BPA in the environment adversely affects human health due to contamination of air, drinking water and food. Growing concerns about the effects of BPA have led to its regulation in production and development of alternative chemicals to BPA, such as bisphenol S (BPS). However, the effects of BPS were not properly tested before its introduction to production and the effects on human reproduction are still unknown. For this reason, it is desirable to test the effect of BPS on mammalian oocyte development. This study hypothesizes that BPS exposure causes inhibition of meiotic maturation of porcine oocytes in vitro. This study aims to investigate the potency of BPS at low concentrations corresponding to normal human exposures to selected porcine oocyte proteins. The results of this study demonstrate the negative effect of BPS on the progression of meiotic maturation and reaching the mature oocyte stage. In addition, the results show an increase in the formation of defective meiotic spindles and a disruption of mitochondrial integrity after exposure to BPS concentrations. However, the effect of BPS on double-strand breaks was not demonstrated in this study, in contrast to the case of BPA. Taken together, the results show...
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Effect of endocrine disruptor bisphenol S on human health and reproduction
Procházková, Bára ; Petr, Jaroslav (advisor) ; Děd, Lukáš (referee)
Bisphenol S (BPS) is an environmental pollutant that has replaced bisphenol A (BPA) in plastic and paper products since 2011 as a safer "BPA-free" alternative. However, due to its rapid replacement, its potential adverse effects have not been investigated in detail, and due to its structural similarity to BPA, many studies suggest that it acts, like BPA, as an endocrine disruptor. Its use is wide and BPS is globally widespread, found in the environment and has been detected in both animal and human tissues. Nevertheless, an overall effect of BPS on an organism remains unclear. This work is focusing on gathering sources for its effect on reproductive ability and its association with metabolic disorders.
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