|
|
Design of optoelectronic sensor for biological tissues
Balík, Zdeněk ; Bartlová, Milada (referee) ; Tománek, Pavel (advisor)
Biological tissues are optically inhomogeneous and absorbent materials. They are comprised of various structures such as cells, cell organelles, and various fiber structures. Due to these structures leads to different interactions with the light and phenomena such as absorption, scattering and polarization of light. Different ultrasonic, electromagnetic, electrical and optical methods are used for meat ageing detection. Muscles are turbid anisotropic media, they exhibit changes in electrical and optical properties according to the direction of the electrical and optical fields in the sample. The diploma work assesses the feasibility of impedance measurements for meat ageing detection and their comparison with optical measurement of scattered polarized light. Two different sample were used: pork chop slices and chicken breasts were used for their relative homogeneity. The electrical method is a promising method due to the possibility of getting much information and realizing cheap and fast enough measurement systems. The proposed optical method allows measure the rotation of polarization plane with meat ageing in the range of 95 degrees within considered period. Through the first promising results obtained in this thesis, a further work has to be provided to determine closer relationship between optical scattering characteristics, electrical anisotropy in ageing-related tissue structural properties.
|
|
|
The use of small punch tests for materials with a higher level of structural inhomogeneity
Gordiak, Michal ; Řehořek, Lukáš (referee) ; Válka, Libor (advisor)
Master's thesis deals with evaluating applicability of correlation relationships between material characteristics determined by Small Punch Test and standard tensile test for material AlSi7Mg0,6 manufactured by casting and technology SLM. Results of Small Punch Tests are correlated with yield strength, tensile strength, elongation, and Young's modulus of elasticity. For each material characteristic various correlation methods are compared, while for each method corresponding coefficients are determined. Consequently, the applicability of individual methods is evaluated by substituting coefficients determined by various studies. Primarily analyzed are correlation methods for which future normalization is expected. The results of master's thesis show that structural inhomogeneity caused by SLM process does not result in high inaccuracies in determining material characteristics. Larger impact on material characteristics has high porosity, which was identified in cast material and led to significant deviations in evaluating tensile strength and elongation.
|
|
|
Manufacturing of a Component by Using Hydroforming Technology and Its Optimization
Harant, Martin ; Peterková, Eva (referee) ; Řiháček, Jan (advisor)
The project deals with analysis and optimization of the geometry of the stamped part produced by the pillow hydroforming. The blank consists of two sheets of steel DC01, which is welded by laser beam. The forming process can cause excessive thinning and cracking of the part. By evaluation of mechanical tests is created material model, which is the basis for numerical simulation created in software PAM-STAMP. The outputs are analysis which provide information about critical points, failure pressure, limiting deformations and prediction of springback. Validity of the numerical simulation is verified by comparison with the experimentally obtained data. The comparative criterions are the failure pressure and the material thickness at various locations. Based on the results of the numerical simulation, the optimization of the geometry is created. The optimization uses different values of radius at the crack point.
|
|
|
Mixed lipids and their interaction with biopolymers
Cigánková, Hana ; Smilek, Jiří (referee) ; Mravec, Filip (advisor)
This thesis focuses on the study of interactions between hyaluronan and lipid DOTAP and its mixtures with DPPC in water. First, the aggregation behavior of the lipid itself and its mixtures was measured, afterwards the influence of hyaluronan in different concentration was studied. Further the fluorescence anisotropy of fluorescence probe DPH in these mixtures and influence of cholesterol was measured. These properties were investigated by fluorescence spectoscopy using pyrene and DPH as fluorescence probes. The measurements revealed that the addition of hyaluronan has observable influence on the aggregation behavior of the lipid DOTAP and its mixtures with DPPC and that cholesterol influences liposomes fluidity.
|
|
|
Microviscosity probes in study of aggregation in a biopolymer-surfactant system.
Vašíčková, Kamila ; Vala, Martin (referee) ; Mravec, Filip (advisor)
The effect of cationic surfactant concentration and ionic strength on anisotropy of fluorescence of probes diphenylhexatrien and fluorescein has been investigated in the system of cationic surfactant and in the system of cationic surfactant and hyaluronan. The investigation has been done by fluorescence emission spectroscopy. Obtained anisotropy gives information about microviscosity of investigated systems. Subsequently the system of cationic surfactant and hyaluronan has been investigated by 9-(2-carboxy-2cyanovinyl)julolidine, 4-(dicyanovinyl)julolidine and 1,3-bispyrenylpropane probes. The information about the microviscosity of the system gives the integral under the emission curve of 9-(2-carboxy2-cyanovinyl)julolidine and 4-(dicyanovinyl)julolidine and the ratio between excimer and monomer of 1,3-bispyrenylpropane. It has been discovered that the ionic strength influences the anisotropy of diphenylhexatrien and fluorescein only in the fist addition of salt (concentration 0,025 mol dm-3) and that addition of hyaluronan influences the anisotropy of diphenylhexatrien and fluorescein only in samples without addition of salt. Results of measurment with 9-(2-carboxy2-cyanovinyl)julolidine and 4(dicyanovinyl)julolidine describe the formation of aggregates of catinoc surfactant with hyaluronan and characterize these aggregates from the point of microviscosity.
|
|
|
Determination of thermal conductivity anisotropy of polymeric heatsinks for electronics
Brachna, Róbert ; Kůdelová, Tereza (referee) ; Komínek, Jan (advisor)
The master's thesis focuses on creating a numerical model of a polymeric heat sink with emphasis on its significant thermal conductivity anisotropy. This anisotropy is caused by highly thermally conductive graphite filler. Its final orientation is given by the melt flow inside the mould cavity during injection molding. The numerical model is created on the basis of a heat sink prototype subjected to experimental measurements, whose physical conditions are reliably replicated by the model. The determination of anisotropy is divided into two parts. The qualitative part is based on the fracture analysis of the heat sink prototype and determines the principal directions of the conductivity tensor in individual sections of the geometry. The computation of principal conductivities falls into the quantitative part, in which this task is formulated as an inverse heat conduction problem. The input data for the proposed task are experimentally obtained temperatures at different places of the geometry. The values of principal conductivities are optimized to minimize the difference between the measured and simulated temperatures.
|
|
|
Numerical solutions of EMC problems of small airplanes
Šeděnka, Vladimír ; Dědková, Jarmila (referee) ; Mazánek, Miloš (referee) ; Raida, Zbyněk (advisor)
Disertace popisuje současné problémy v certifikaci malých letadel, které by se měly v budoucnu řešit numerickým modelováním. Tento postup má zefektivnit návrh a zlevnit certifikaci letadel. Práce je úzce spjata s projektem HIRF-SE, který se problematikou certifikace letadel numerickými metodami zabývá. Podstatná část práce je věnována popisu dvou modulů pro platformu HIRF-SE: řešič BUTFE založený na metodě konečných prvků v časové oblasti a budicí nástroj BUTFE_EXC. Práce popisuje řešení pohlcujících okrajových podmínek, modelování disperzních a anizotropních materiálů a aproximaci tenkých drátů. Speciální pozornost je věnována řešení aproximace tenkých drátů s ostrými ohyby, jejíž současná formulace způsobuje překryvy mezi jednotlivými segmenty drátu.
|
|
|
Analysis of direction dispersion of collagen fibers in soft tissues
Sádovská, Terézia ; Lisický, Ondřej (referee) ; Turčanová, Michaela (advisor)
The bachelor thesis deals with the directional distribution of collagen fibres in soft tissues especially in arteries and tendons. The first part gives basic information about anatomy of human vascular system, particulary structure of the arteries. There are explained polarization microscopy principle of materials exhibiting birefringence. Further is described relation between direction of density and mechanical properties expresses as modulus in tensile. In the next section, we will be defined stress in arterial wall using linear stregth and stiffness. In the last part of thesis will be observed dispertion of collagen fibres in tendon. This dispertion is described by circular distribution Von Mises. At the end is evaluated how dispertion of collagen fibres effects mechanical properties of arteries.
|
|
|
Interaction of negatively charged vesicular systems based on ionic amphiphilic pairs with polycations
Fardová, Lucie ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This work is focused on the preparation of negatively charged vesicular systems from amphiphilic pairs deprived of counterions and their interaction with the polycation. First, vesicular systems were prepared from the surfactants sodium dodecyl sulfate and cetyltrimethylammonium bromide. The addition of cholesterol and phosphatidic acid stabilized and at the same time gained a negative charge. Subsequently, there was an interaction with the polycation diethylaminoethyl-dextran hydrochloride. The systems were investigated by the measuring of particle size, zeta potential, generalized polarization and anisotropy. The measurement of generalized polarization was enabled with the laurdan probe and measurement of anisotropy was performed with the introduction of the 1,6-diphenyl-1,3,5-hexatriene probe. The results indicate the successful preparation of stable, negatively charged systems that interacted with positive diethylaminoethyl-dextran hydrochloride, which affected the behavior of the outer membrane but not the inner. The systems were still stable.
|
|
|
Computational Modelling of Mechanical Behaviour of "Elastomer-Steel Fibre" Composite
Lasota, Tomáš ; Okrouhlík,, Miloslav (referee) ; Nováček,, Vít (referee) ; Burša, Jiří (advisor)
Tato práce se zabývá výpočtovými simulacemi zkoušek jednoosým tahem a tříbodovým ohybem kompozitního vzorku složeného z elastomerové matrice a ocelových výztužných vláken orientovaných pod různými úhly, jakož i jejich experimentální verifikací. Simulace byly provedeny pomocí dvou různých modelů - bimateriálového a unimateriálového výpočtového modelu. Při použití bimateriálového modelu, který detailně zohledňuje strukturu kompozitu, tzn. pracuje s matricí a jednotlivými vlákny, je zapotřebí vytvořit model každého vlákna obsaženého v kompozitu, což přináší řadu nevýhod (pracná tvorba výpočtového modelu, řádově větší množství elementů potřebných k diskretizaci v MKP systémech a delší výpočetní časy). Na druhé straně v unimateriálovém modelu se nerozlišují jednotlivá vlákna, pracuje se pouze s kompozitem jako celkem tvořeným homogenním materiálem a výztužný účinek vláken je zahrnut v měrné deformační energii. Porovnání experimentů se simulacemi ukázalo, že bimateriálový model je v dobré shodě s experimenty, na rozdíl od unimateriálového modelu, který je schopen poskytnou odpovídající výsledky pouze v případě tahového namáhání. Z tohoto důvodu byl hledán způsob, který by umožnil rozšířit unimateriálový model o ohybovou tuhost výztužných vláken. V roce 2007 Spencer a Soldatos publikovali rozšířený unimateriálový model, který je schopen pracovat nejen s tahovou, ale i ohybovou tuhostí vlákna. Představený obecný model je však založen na Cosseratově teorii kontinua a jeho praktické využití je pro jeho složitost nemožné. Proto byl vytvořen zjednodušený model (částečně podle Spencera a Soldatose) s vlastní navrženou formou měrné deformační energie. Za účelem ověření nového unimateriálového modelu s ohybovou tuhostí vláken byly odvozeny všechny potřebné rovnice a byl napsán vlastní konečno-prvkový řešič. Tento řešič je založen na Cosseratově teorii kontinua a obsahuje zmíněný anizotropní hyperelastický unimateriálový model zahrnující ohybovou tuhost vláken. Vzhledem k tomu, že v případě Cosseratovy teorie jsou při výpočtu potřebné i druhé derivace posuvů, bylo nutné použít tzv. C1 prvky, které mají spojité jak pole posuvů, tak jejich prvních derivací. Nakonec byly provedeny nové simulace s využitím vlastního řešiče, které ukazují, že tuhost vláken lze u nového unimateriálového modelu řídit odpovídající materiálovou konstantou. V závěru práce je pak diskutováno, zda je nový unimateriálový model s ohybovou tuhostí schopen poskytnout stejné výsledky jako model bimateriálový, a to jak při tahovém tak i ohybovém namáhání kompozitního vzorku.
|
guest ::
