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Characterization of eukaryotic translation initiation factor 3 subunits (eIF3) in A. thaliana male gametophyte
Linhart, Filip ; Hafidh, Said (advisor) ; Retzer, Katarzyna (referee)
From RNA-to-protein, translation initiation and protein synthesis is mediated by trans-acting factors that recognize mRNA features common to almost all eukaryotes. Eukaryotic translation initiation factor 3 complex (eIF3) is a highly conserved protein complex that recognizes 5'-CAP elements of the mRNA to initiate translation. eIF3 consists of nine subunits, three of them having two isoforms: eIF3A, eIF2B1, eIF3B2, eIF3C1, eIF3C2, eIF3D, eIF3E, eIF3F, eIF3G1, eIF3G2, eIF3H and eIF3K. This work deals with functional characterization, expression and subcellular localization of eIF3B1, eIF3B2 and eIF3E in Arabidopsis thaliana male gametophyte and interaction of eIF3E with the Constitutive photomorphogenesis 9 (COP9) complex as a regulatory complex of eIF3E post-translational control. Here we show that depletion of eif3b1 or eif3b2 is not gametophytic lethal and that the two protein might function redundantly, whereas, knockout of eIF3E causes male gametophyte lethality. Interestingly, eif3b1 show post-fertilization defects during embryogenesis, suggesting that its redundancy with eIF3B2 is restricted to the gametophyte. Gene expression studies revealed high expression of eIF3 subunits in actively dividing zones of leaf primordia, root meristem and root elongation zones as well as in the vegetative...
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Reaction of selected varieties of winter oilseed rape (Brassica napus) on the application of fungicides
Vojík, Jan ; Bečka, David (advisor) ; Michal, Michal (referee)
Oilseed rape i sone of the most important crops in the world. I tis the most important oil crop in Czech Republic. I the last 50 years it had undergone large change in breedeng. Ratio of grown hybrids at present tops the open pollinated lines ( planning for harvest in 2016 saw 88% hybrids and only 12% of lines). There are quite important differences between varieties in tolerance to fungal diseases and not only to them. There are first varieties marketed with gene of pot shatter tolerance and others. The pressure of fungal diseases grows due to growing planting area of the rape.
Trials with oil seed rape were established in the growing season 2014/15 on four varieties and two locations. We tested following hybrids: Arsenal (Limagrain), DK Exstorm (Monsanto), Jumper (Bayer), SY Cassidy (Syngenta). The locations were in the district Jičín farm AGRO Slatiny a.s., and in the district Kolín farm ZD Křečhoř a.s.. On each location were planted two trial variants 1) without fungicide application 2) with the application of fungicides. On the location Slatiny were applied three fungicides (Toprex, Paroli, Propulse), on the location Křečhoř also three fungicides (Toprex, Magnello, Amistar XTRA).
The aim of the bachelor study was to test growth yield and qualitative parameters of the varieties together with observation of fungal diseases and their influence on yield and oil content. We were testing the infestation of following diseases Phoma lingam, Sclerotinia sclerotiorum, Verticilium dahlie Botrytis cinerea.
Hybrid DK Exstorm (195cm) was the tallest variety on both locations followed by hybrid Arsenal (193,5 cm). By number of branches was the best ranked variety hybrid Arsenal, the lowest was SY Cassidy. On fungicide treated variants grew number of pods per m2 by average 6% on both locations. Average yield vas slightly higher in location Křečhoř in both variants, treated (70kg) and untreated (50 kg). On both locations was visible difference between treated and untreated variants. Yield increase was in average 5,4% (200kg/ha). Varieties with best reaction to fungicide treatment were DK Exstorm (Monsanto) and Jumper (Bayer). Fungicide application influenced also crop height, number of branches and pods per m2.
Following conclusions are made in the bachelor study:
- Fungicide application increased yield in average by 5,4% (i.e.200 t/ha) and improwed the health of the crop.
- DK Exstorm and Jumper were varieties with the best reaction on the fungicide treatmentArsenal (4,37 t/ha) was the best yileding variety on both locations followed by hybrid Jumper (4,27 t/ha).
- Arsenal is the recomended variety for both locations Křečhoř and Slatiny
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