National Repository of Grey Literature 105 records found  previous11 - 20nextend  jump to record: Search took 0.01 seconds. 
Assessment of PHB effects at organismal and sub-organismal levels through E. fetida.
Demová, Kristína ; Řezáčová, Veronika (referee) ; Zlámalová Gargošová, Helena (advisor)
The use of biodegradable plastics, which are competitive with synthetic plastics and have the ability to be completely degraded by micro-organisms, is gaining ground. In real life conditions, biodegradation does not always proceed as expected, and these plastic particles may persist in the environment for long periods of time. Therefore, the verification of the safety of these plastics is the subject of many studies. This was also the aim of the present thesis, which deals with the impact of poly-3-hydroxybutyrate microparticles (representative of biodegradable plastics) on the organism Eisenia fetida (representative of soil organisms). Its effects were assessed using classical ecotoxicological tests. Methods to observe effects at the sub-organism level - using selected biomarkers - were also introduced and implemented. From the obtained results, no significant effect of the investigated biodegradable plastic on the test organism E. fetida was demonstrated, but the introduced methods can be used in the future to obtain more results to assess the effect at the sub-organismal level of the organism in a relevant way.
The use of ecotoxicity tests for the use of treatment technologies
Konečná, Kateřina ; Komendová, Renata (referee) ; Zlámalová Gargošová, Helena (advisor)
This diploma thesis is focused on the assessment of the effectiveness of treatment technologies in domestic wastewater treatment plants. For these purposes, samples were taken from 3 different domestic wastewater trearment plant. Two representatives of the aquatic ecosystem, the plant Lemna minor and the freshwater crustacean Thamnocephalus platyurus, were selected for the ecotoxicity assessment. For assessment from an analytical point of view, indicators were selected according to the Government Regulation on the discharge of water into surface and underground waters No. 401/2015 Coll. and 57/2016 Coll. All the selected residential cleaning plants correspond in size to 30–500 equivalent inhabitants, while they are used by approx. 10–20 inhabitants. The results of ecotoxicological tests confirmed that domestic wastewater treatment plants effectively reduce the level of toxicity. In all domestic waste water treatment plants, IC50 values for L. minor and LC50 values for T. platyurus were determined only at the influent, as neither inhibition nor mortality higher than 50% compared to the control group was achieved at the effluent. In L. minor, the growth inhibition values (IC50) on the influent were in the range of 9.39–43.99 ml/l, in T. platyurus the LC50 values in the influent were determined in the range of 5.6–20 ml/l. However, the results of the chemical analysis revealed that none of the water from the domestic treatment plants selected by us is suitable for discharge into groundwater or surface water, as they exceed the limit values set in government regulations.
Use of terrestrial gastropods in ecotoxicity tests
Fojt, Pavel ; Komendová, Renata (referee) ; Kráčmar, Stanislav (referee) ; Zlámalová Gargošová, Helena (advisor)
The subject of this disertation is the use of terrestrial gastropods in ecotoxicity tests. Specifically, the use of a cosmopolitan terrestric gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum). The whole life cycle of this snail is very closely related the soil ekosystem. Here, the snails participates in some of the very important processes like the decomposition of the dead organic matter. This work is focused on the use of these snails to evaluate potential negative effect of some selected elements, which could, in different volumes, enter the soil ecosystem. The next task is to divide the ecotoxicity tests to different categories, depending on the life stage of this organism and then to compare the sensitivity of those life stages to different levels of contamination by certain pollutants.
Study on the influence of plasma activated water on seed germination
Vozár, Tomáš ; Zlámalová Gargošová, Helena (referee) ; Kozáková, Zdenka (advisor)
This bachelor thesis deals with study on the influence of plasma activated water on wheat seed germination. After interaction with the plasma, the water changes its composition and obtain new properties that are useful in agriculture. The theoretical part is divided into two parts. The first part of the theory is dealing with basic knowledge about plasma, its properties, and possibilities of plasma generation in laboratory conditions. In the second part, the work deals with plasma activated water – PAW. The preparation of plasma activated water is described in details as well as its physical-chemical properties and composition with respect to the PAW possible applications. The aim of the experimental part of this work was to prepare plasma activated water in three different plasma systems and apply it on wheat seeds. These three ways of activation used direct and indirect interaction of plasma with water surface. After the seeds were germinated, the influence of plasma activated water on seed germination and quality of crops was observed and compared to control samples. The influence of different ways of water activation was also evaluated. Results show positive effects of plasma activated water on the seed germination and it also increased the quality of crops. Next steps in this research should be the transfer of this experiment from the laboratory scale on the Petri dish to the pot experiments in soil.
Ecotoxicological evaluation of biochar via organism Folsomia candida
Marcinko, Juraj ; Procházková, Petra (referee) ; Zlámalová Gargošová, Helena (advisor)
The aim of this bachelor thesis was to ecotoxicologically evaluate selected biochar samples and to assess, using a test organism, the possible long-term and short-term effects on soil invertebrates. One of the possible uses of biomass is to convert it into biochar with subsequent application to soil. Soil fauna may be affected after biochar application, therefore ecotoxicological tests in a contact arrangement were chosen. Folsomia candida was used as the test organism. Short-term effects were recorded through escape tests, specifically low abundance of organisms in samples (I) and (T). Significant preference of organisms was observed for sample (O). Samples (I) and (T) had a further impact on the survival of organisms and on the quality of reproduction, where in some cases this caused a resolute impact. Sample (O), at different concentration levels, had no effect on the test organisms. The results of these tests approximate how soil invertebrates could be affected after biochar application.
Risks associated with the use of biochar in the terrestrial ecosystem
Valeeva, Adeliia ; Řezáčová, Veronika (referee) ; Zlámalová Gargošová, Helena (advisor)
The aim of this bachelor thesis was to assess the ecotoxicity of biochar produced from sewage sludge in Ingolstadt. Biochar is one of the carbon storage options and it’s applied to the soil. Biochar has an effect not only on the terrestrial ecosystem in direct contact of organisms with the sample, but also on aquatic in the leaching of substances from biochar into water. Therefore, contact and aquatic tests were chosen to assess the ecotoxicological effect. The representative of the decomposers was the earthworm Eisenia fetida, the primary producer – the plant Lactuca Sativa and the Daphnia magna was chosen from the aquatic organisms. The test results differed slightly, but the lowest negative effect on the test organisms was shown by soil samples with the addition of 5 g/kg and 10 g/kg granular biochar.
Determination of antibiotics in the soil ecosystem
Brož, Tomáš ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
This work deals with a current issue of increasing drug concentrations and thus the growing number of resistant bacteria in the soil. One of the problem helping this issue is the fertilization of agricultural fields with animal faeces, in which antibiotics occur in an unchanged and still active form, as well as the insufficient effectiveness of conventional methods used in wastewater treatment plants. In an effort to improve the monitoring of this problem, a method for the determination of these antibacterial agents in the soil matrix, using solid phase extraction, and also a UPLC/MS method for their evaluation have been developed and optimized. From the group of sulfonamide antibiotics, sulfacetamide, sulfadiazole, sulfamerazine, sulfamethazine, sulfamethoxazole, sulfapyridine, sulfasalazine, sulfathiaosal and trimethoprim were selected. The lowest limit of detection for the method is 0.3 µg/g for sulfathiazole and the highest of 8.1 µg/g for sulfasalazine. The highest average yields were achieved with sulfamethoxazole, namely 63 ±8 %.
Study of the effect of myocardial fibrosis on elemental distribution in rat soft tissues using Laser Ablation ICP-MS
Machalová, Martina ; Zlámalová Gargošová, Helena (referee) ; Vašinová Galiová, Michaela (advisor)
The most common cause of death in the Czech Republic continue to be cardiovascular diseases. The field of heart failure therapy still keeps moving forward. Yet myocardial fibrosis is one of the consequences and causes of heart failure for which there exists no effective medical care. The solution would be predicting the advancement of fibrosis and a preventive therapy. Many medical methods are being developed now in this regard, and the element distribution in affected tissue could help them. That is the reason why this work centers on the research of the element distribution in rat hearts with an induced myocardial fibrosis. An influence of sample couloration on the analysis was observed during its course. Special attention was paid to the distribution of iron in the fibrotic tissue as well as to the comparisson of the images of myocardial fibrosis. It seems that the iron distribution specificly could be helpful in recognising a damaged myocardium.
Use of the QuEChERS method for the analysis of fluoroquinolone antibiotics in soil
Brabcová, Kristýna ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
The content of this master's thesis is an application of frequently used extraction method QuEChERS on soil or other samples like litter or manure containing fluoroquinolone antibiotics. In the theoretical part of this thesis are chapters describing drugs, antibiotics and antibiotic resistance. Fluoroquinolones are described in more details with their properties, appearance and behaviour in the environment. The last chapter is focused on analytical methods. In the experimental part of this thesis is chosen in accordance with available publications the most suitable extraction method. This method is tested and optimized on soil samples. The optimized method is applied on the real soil and poultry litter samples to determine the amount of ciprofloxacine and enrofloxacine. This extraction method is used on other fluoroquinolone antibiotics (norfloxacine, ofloxacine, pefloxacine, moxifloxacine) in soil. All of the samples were analyzed by LC-MS.
Determination of antibiotic residues in soil
Kaprinay Bréda, Boglárka ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
This master‘s thesis is focused on the optimization of sample preparation and analytical method for the determination of fluoroquinolone. The theoretical part describes the principles of analysis in soil samples, environmental aspects of antibiotic resistance, characteristics of UPLC-MS / MS methods, description of quinolone. The experimental part deals with the identification and determination of fluoroquinolone substance (ciprofloxacin, enrofloxacin) in soil in which manure from poultry litter was incorporated using liquid chromatography with mass spectrometry (MS / MS, triple quadrupole). Internal standard and standard addition methods were used to quantify ciprofloxacin and enrofloxacin. The amount of CIP in the samples ranged from 0.2030 to 12.7088 µg·g-1 and for ENR from 0.2537 to 10.0224 µg·g-1.

National Repository of Grey Literature : 105 records found   previous11 - 20nextend  jump to record:
Interested in being notified about new results for this query?
Subscribe to the RSS feed.