National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Methods for viability evaluation of Xanthomonas vesicatoria after low temperature plasma treatment
ZEMANOVÁ, Marta
The thesis deals with methods for viability evaluation of the phytopathogenic bacterium Xanthomonas euvesicatoria after low-temperature plasma treatment. Low-temperature plasma produced by Gliding Arc experimental device was used for treatment of X. euvesicatoria. The viability of the bacterial cells was assessed using a scanning electron microscope (SEM) and by measuring of the fluorescence in the Smart-DART device using PrestoBlue chemical reagent. Methodology has been optimised for the sample preparation for the treatment by low temperature plasma and used for evaluation of applied methods. Lethal effect of gliding arc plasma to this gram-negative bacteria was verified by SEM which showed. There is significant structural changes on the cell surface. Viability assessment of X. euvesicatoria using Smart-DART device is a fast, time-saving and inexpensive evaluation of cell viability. The great advantage of this device is its ability to measure the fluorescence in real time. The disadvantage of this method is lower reliability in current stage of research.
Monitoring of phytopathogenic bacteria viability after low-temperature plasma treatment
ZEMANOVÁ, Marta
The bachelor´s thesis is focused on the monitoring of phytopathogenic bacteria´s viability after low-temperature plasma treatment. Gliding Arc device has been used for the plasma treatment of phytopathogenic bacteria. Evaluation was carried out using chemical reagent PrestoBlue which was followed by spectrophotometric evaluation and calculating CFU/ml from the regression equation derived from the calibration curve. The effect of the low-temperature plasma on the viability of plant pathogenic bacteria was prooved. Gram-negative bacteria Erwinia amylovora and Rahnella aquatilis were the most susceptible to the plasma treatment. These phytopathogenic bacteria belong to the family Enterobacteriaceae. Representatives of gram-positive bacteria showed lower sensitivity to the low-temperature plasma treatment. Suitable method has also been designed for the treatment of phytopathogenic bacteria by the low-temperature plasma at atmospheric pressure.

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5 ZEMANOVÁ, Michaela
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1 Zemanová, Magdaléna
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14 Zemanová, Martina
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2 Zemanová, Miroslava
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