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Study of common bean drought response with the use of leaf optical properties
Svitáková, Lenka ; Lhotáková, Zuzana (advisor) ; Zámečník, Jiří (referee)
The negative effects of climate change have affected the conditions of agriculture areas, which leads to hampered cultivation of crops, including the common bean's cultivation. Changes, such as extreme temperature swings and lowered water availability in soil create a big challenge for today's agriculture in seeking solutions for safeguarding the food security for all people on our planet. The aim of this thesis was to study a wider range of genotypes of common bean to quantify their resistance against lowered water availability in soil, and to establish new approaches for detecting drought stress with the use of leaf optical properties. From the methodological point of view, this thesis connects the leaf optical properties with anatomical and biophysical leaf traits. The common bean (Phaseolus vulgaris) and the tepary bean (Phaseolus acutifolius) were employed as the model organisms. Plants were cultivated in two differing environments - in a greenhouse setting at the Faculty of Science of the Charles University in Prague and on the experimental fields in Colombia at the international research institute for tropical agriculture - Alliance of Biodiversity International and CIAT. There were 48 genotypes included into the field experiments in Colombia. These included genotypes from both Mesoamerican...
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Methodology of cryoconservation of in vitro cultures of fruit trees
Bilavčík, Alois ; Faltus, Miloš ; Zámečník, Jiří
The purpose of the methodology is to store selected genotypes of fruit trees, especially apple and pear trees, in conditions of cryogenic temperatures within the National Program. The newly created methodology will enable efficient and effective cryopreservation of genetic resources of selected fruit trees. The methodology brings newly defined encapsulation dehydration procedures of cryopreservation optimized using the thermal method of differential scanning calorimetry.
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Immunomodulation effects of the hyperthermia in cancer treatment
Kubeš, Jiří ; Rosina, Jozef (advisor) ; Vrba, Jan (referee) ; Zámečník, Jiří (referee)
The dissertation thesis "Immunomodulation effects of the hyperthermia in cancer treatment" relates on the hyperthermia as evolving method of the cancer treatment. The first part describes hyperthermia in general, especially physical principles of the heating, the equipment necessary for clinical practice of the hyperthermia, basic effects in biological tissues, clinical indications and results. The second part is brief review of mechanisms antitumor immunological response, with emphasis on antigenicity of tumor cells and effector cells in antitumor response.
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Apple dormant bud cryopreservation: Methodology
Bilavčík, Alois ; Faltus, Miloš ; Zámečník, Jiří
In the Czech Republic, ex situ and in vitro conservation is used for apple tree germplasm conservation. In relation to the strategy of maintenance of broad genetic potential of apple tree, there is a necessity to preserve genotypes by storage at ultralow temperatures – cryoconservation. Based on broad spectrum of apple cultivars, an optimised dormant bud cryopreservation protocol was developed. The developed cryoprotocol enables to establish a safe backup storage system for ex situ and in vitro collections of apple tree germplasm. The Ministry of Agriculture of the Czech Republic is the user of this methodology and it will utilize it in the framework of “National Programme on Conservation and Utilization of Plant, Animal and Microbial Genetic Resources for Food and Agriculture”.
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Business Development Project
Weiserová, Gabriela ; Zámečník, Jiří (referee) ; Bartoš, Vojtěch (advisor)
The bachelor thesis deals with the creation of a business plan for the establishment of private nursery school as a limited liability company. The aim of this work is to create a business plan, which could be used as a basis for the real establishment of the company. It is therefore necessary to choose adequate methods for its processing. These will deal with the part of the Own Proposals solution, following the theoretical part, which will introduce the basic theoretical knowledge of the given issue.
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Cryopreservation of sensitive potato genotypes
Faltus, Miloš ; Bilavčík, Alois ; Zámečník, Jiří
This methodology describes the preparation of explants prior to cryopreservation, dehydration of shoot tips, procedure and principles of sample cryopreservation, regeneration of control plants, including evaluation of explant survival and regeneration for sensitive potato genotypes which were formerly very difficult to cryopreserve. Ministry of agriculture of the Czech Republic is the user of this methodology and it applies it in the framework of the "National program for conservation and utilization of plant genetic resources, animals and micro-organisms important for nutrition and agriculture".
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Immunomodulation effects of the hyperthermia in cancer treatment
Kubeš, Jiří ; Rosina, Jozef (advisor) ; Vrba, Jan (referee) ; Zámečník, Jiří (referee)
The dissertation thesis "Immunomodulation effects of the hyperthermia in cancer treatment" relates on the hyperthermia as evolving method of the cancer treatment. The first part describes hyperthermia in general, especially physical principles of the heating, the equipment necessary for clinical practice of the hyperthermia, basic effects in biological tissues, clinical indications and results. The second part is brief review of mechanisms antitumor immunological response, with emphasis on antigenicity of tumor cells and effector cells in antitumor response.
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